Environmental Biotechnology and Genomics Division, CSIR-National Environmental Engineering Research Institute, Nehru Marg, Nagpur, 440020, India.
Department of Forest Biology, Kasetsart University, Bangkok, Thailand.
Mol Biol Rep. 2021 May;48(5):4487-4495. doi: 10.1007/s11033-021-06469-9. Epub 2021 Jun 11.
Bamboo is a non-timber forest product and one of the most important grass plants of industrial and domestic use. It is widely distributed in tropical countries including India, China and Southeast Asian countries with wide genetic diversity. The diversity in the available genotypes becomes an important resource for the selection and improvement of the plants for ecological and commercial use. This study investigates eight commercially and ecologically important bamboo species of six genera (Bambusa, Dendrocalamus, Thyrsostachys, Vietnamosasa, Cephalostachyum and Indocalamus) from India, Thailand and Laos. These were evaluated for genetic differences by molecular makers, chemo-morphological variation and ability of silicon accumulation. The genetic cluster analyses of eight RAPD primers revealed genetic similarities in the ranges of 24-55%. The total silica content varied from 18.34 to 40.08 ppm in leaves of different bamboo species. Chemical analysis of the silicon content by ICP-OES and secondary metabolite profiling on TLC depicted the prominent distinction among the species. The PCA analysis of quantitative morphological data grouped the species in two major clusters and found to correlate with chemical pattern and genetic similarity to some extent. This is the first report that summarizes species-specific variability of leaf silica content, secondary metabolites, and quantitative morphological data towards delineation of genetic phylogeny of bamboo species.
竹子是一种非木材林产品，也是工业和家庭用途最重要的草类植物之一。它广泛分布于印度、中国和东南亚等热带国家，具有广泛的遗传多样性。现有基因型的多样性成为生态和商业用途植物选择和改良的重要资源。本研究调查了来自印度、泰国和老挝的 6 属 8 种具有商业和生态重要性的竹种（Bambusa、Dendrocalamus、Thyrsostachys、Vietnamosasa、Cephalostachyum 和 Indocalamus）。这些竹种通过分子标记、化学生态型变异和硅积累能力进行了遗传差异评估。8 个 RAPD 引物的遗传聚类分析显示遗传相似性在 24-55%之间。不同竹种叶片中的总硅含量从 18.34 到 40.08ppm 不等。通过 ICP-OES 对硅含量的化学分析和 TLC 上的次生代谢产物分析显示出物种之间的明显区别。定量形态数据分析的 PCA 分析将物种分为两个主要聚类，并在一定程度上与化学模式和遗传相似性相关。这是第一份总结竹叶硅含量、次生代谢物和定量形态数据的物种特异性变异性，以描绘竹种遗传系统发育的报告。
