Department of Pathology and Pathophysiology, School of Medicine & Holistic Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing, China.
Department of Microbiology and Immunology, Medical School, Southeast University, Nanjing, China.
Front Immunol. 2021 May 26;12:675822. doi: 10.3389/fimmu.2021.675822. eCollection 2021.
We have reported that tumor-derived autophagosomes (DRibbles) were efficient carriers of tumor antigens and DRibbles antigens could be present by DRibbles-activated B cells to stimulate effect and naïve T cells in mice. However, the effect of DRibbles on human B cells remains unclear. Herein, we found that DRibbles can also efficiently induce proliferation and activation of human B cells and lead to the production of chemokines, cytokines and hematopoietic growth factors. We further demonstrated human B cells can effectively phagocytose DRibbles directly and cross-present DRibbles antigens to stimulate antigen-specific memory T cells. Furthermore, we found that membrane-bound high-mobility group B1 (HMGB1) on DRibbles was crucial for inducing human B cells activation. Therefore, these findings provide further evidence to promote the clinical application of B-DRibbles vaccines.
我们曾报道过，肿瘤来源的自噬体（DRibbles）是肿瘤抗原的有效载体，DRibbles 抗原可通过 DRibbles 激活的 B 细胞呈递，从而刺激小鼠中的效应和初始 T 细胞。然而，DRibbles 对人 B 细胞的影响尚不清楚。在此，我们发现 DRibbles 还可以有效地诱导人 B 细胞的增殖和活化，并导致趋化因子、细胞因子和造血生长因子的产生。我们进一步证明，人 B 细胞可以有效地直接吞噬 DRibbles，并交叉呈递 DRibbles 抗原，以刺激抗原特异性记忆 T 细胞。此外，我们发现 DRibbles 上的膜结合高迁移率族蛋白 B1（HMGB1）对于诱导人 B 细胞的活化至关重要。因此，这些发现为促进 B-DRibbles 疫苗的临床应用提供了进一步的证据。
