Thudi Mahendar, Chen Yinglong, Pang Jiayin, Kalavikatte Danamma, Bajaj Prasad, Roorkiwal Manish, Chitikineni Annapurna, Ryan Megan H, Lambers Hans, Siddique Kadambot H M, Varshney Rajeev K
Center of Excellence in Genomics and Systems Biology, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India.
The UWA Institute of Agriculture, The University of Western Australia, Perth, WA, Australia.
Front Plant Sci. 2021 May 28;12:636973. doi: 10.3389/fpls.2021.636973. eCollection 2021.
Chickpea-the second most important grain legume worldwide-is cultivated mainly on marginal soils. Phosphorus (P) deficiency often restricts chickpea yields. Understanding the genetics of traits encoding P-acquisition efficiency and P-use efficiency will help develop strategies to reduce P-fertilizer application. A genome-wide association mapping approach was used to determine loci and genes associated with root architecture, root traits associated with P-acquisition efficiency and P-use efficiency, and any associated proxy traits. Using three statistical models-a generalized linear model (GLM), a mixed linear model (MLM), and a fixed and random model circulating probability unification (FarmCPU) -10, 51, and 40 marker-trait associations (MTAs), respectively were identified. A single nucleotide polymorphism (SNP) locus (Ca1_12310101) on Ca1 associated with three traits, i.e., physiological P-use efficiency, shoot dry weight, and shoot P content was identified. Genes related to shoot P concentration (NAD kinase 2, dynamin-related protein 1C), physiological P-use efficiency (fasciclin-like arabinogalactan protein), specific root length (4-coumarate-CoA ligase 1) and manganese concentration in mature leaves (ABC1 family protein) were identified. The MTAs and novel genes identified in this study can be used to improve P-use efficiency in chickpea.
鹰嘴豆是全球第二重要的食用豆类，主要种植在边际土壤上。磷（P）缺乏常常限制鹰嘴豆产量。了解编码磷获取效率和磷利用效率性状的遗传学将有助于制定减少磷肥施用的策略。采用全基因组关联作图方法来确定与根系结构、与磷获取效率和磷利用效率相关的根系性状以及任何相关的代理性状相关的基因座和基因。分别使用三种统计模型——广义线性模型（GLM）、混合线性模型（MLM）和固定与随机模型循环概率统一法（FarmCPU）——鉴定出了10个、51个和40个标记-性状关联（MTA）。在Ca1上鉴定出一个与三个性状相关的单核苷酸多态性（SNP）位点（Ca1_12310101），即生理磷利用效率、地上部干重和地上部磷含量。鉴定出了与地上部磷浓度相关的基因（NAD激酶2、动力蛋白相关蛋白1C）、生理磷利用效率相关基因（类成束蛋白阿拉伯半乳聚糖蛋白）、比根长相关基因（4-香豆酸-CoA连接酶1）和成熟叶片中锰浓度相关基因（ABC1家族蛋白）。本研究中鉴定出的MTA和新基因可用于提高鹰嘴豆的磷利用效率。
