用于测定尿液中前列腺素F和E型代谢物的双同位素衍生稀释法。

Double isotope derivative dilution method for the determination of prostaglandin F and E type metabolites in urine.

作者信息

Aizawa Y, Yamada K, Hata M

出版信息

Prostaglandins. 1977;14(6):1165-74. doi: 10.1016/0090-6980(77)90293-3.

Abstract

This method was developed for the quantitative determination of the main ureinary metabolite (MUM) of prostaglandin (PG) F and E type in man or experimental animal. This method was based on the extraction of PG-MUM after addition of 3H-PG-MUM, the esterification with 14C-acetic anhydride and the separation and purification by column chromatography. Excretion values of adult men were PGF-MUM 1.46--15.01 microgram/8 hr (n=11), PGE-MUM 0.8*--15.42 microgram/8 hr; and with women, PGF-MUM 0.41--7.75 microgram/8 hr (n=11), PGE-MUM 0.60--11.39 microgram/8 hr.

摘要

该方法用于定量测定人或实验动物中前列腺素（PG）F型和E型主要尿代谢物（MUM）。该方法基于加入³H-PG-MUM后提取PG-MUM，用¹⁴C-乙酸酐进行酯化，并通过柱色谱进行分离和纯化。成年男性的排泄值为PGF-MUM 1.46--15.01微克/8小时（n = 11），PGE-MUM 0.8*--15.42微克/8小时；女性的排泄值为PGF-MUM 0.41--7.75微克/8小时（n = 11），PGE-MUM 0.60--11.39微克/8小时。