Husz Taylor C, Smith Wyatt N, Lockard Caleb G, Homolka Megan N, Anderson Peter T, Gentry Wes W, Sugg Joel D, Casey Kenneth D, Jennings Jenny S
Texas A&M AgriLife Research, Amarillo, TX 79106, USA.
Midwest PMS, LLC, Firestone, CO 80504, USA.
Transl Anim Sci. 2021 May 16;5(2):txab090. doi: 10.1093/tas/txab090. eCollection 2021 Apr.
The objective of this study was to evaluate the ruminal fermentation characteristics of ruminally fistulated beef steers consuming a steam-flaked corn () or dry-rolled corn () based diet containing either Rumensin 90 (; Elanco, Greenfield, IN), or Monovet 90 (; Huvepharma, Peachtree City, GA). Six ruminally fistulated steers (657.7 kg ± 72.6) housed individually were used in a 6 × 6 Latin square design with 2 × 3 factorial treatment arrangement. Each of the 6 periods were 15 d with 14 d for diet adaptation and 1 d of rumen fluid collections. Dietary treatments were DRC without monensin sodium (), SFC without monensin sodium (), DRC with Rumensin 90 (), DRC with Monovet 90 (), SFC with Rumensin 90 (), and SFC with Monovet 90 (). Rumen contents and fluid were collected through the fistula of each animal at 0, 3, 6, 12, and 24 h on d 15 of each period. Rumen fluid collected at 6 h post-feeding each period was used for in vitro analyses. Steer was the experimental unit and the model included fixed effects of grain processing, additive, and grain processing × additive. Total gas produced was composited from each in vitro bottle into a gas collection bag for the 48-h determination of methane concentration. No differences were detected for DMI ( = 0.81). Ruminal pH did not differ for the control or additive treatments ( = 0.33). However, ruminal pH was lower ( < 0.01) with SFC compared to DRC. There was a significant difference in acetate to propionate ratio for grain type ( = 0.01) and a tendency for additive inclusion ( = 0.06). Additive inclusion reduced methane proportion of total gas compared to control treatments ( ≤ 0.01). Overall, monensin sodium reduced methane concentration though source had no effect on DMI or ruminal pH.
本研究的目的是评估瘤胃造瘘的育肥牛在采食基于蒸汽压片玉米()或干碾压玉米()且添加了瘤胃素90(;美国伊利诺伊州格林菲尔德市礼来公司)或莫能菌素90(;美国佐治亚州桃树市胡维制药公司)的日粮时的瘤胃发酵特性。选用6头单独饲养的瘤胃造瘘育肥牛(657.7千克±72.6),采用6×6拉丁方设计,进行2×3析因处理安排。6个试验期各为15天,其中14天用于日粮适应,1天用于采集瘤胃液。日粮处理包括不添加莫能酸钠的干碾压玉米()、不添加莫能酸钠的蒸汽压片玉米()、添加瘤胃素90的干碾压玉米()、添加莫能菌素90的干碾压玉米()、添加瘤胃素90的蒸汽压片玉米()以及添加莫能菌素90的蒸汽压片玉米()。在每个试验期的第15天,于0、3、6、12和24小时通过每头动物的瘘管采集瘤胃内容物和瘤胃液。每个试验期采食后6小时采集的瘤胃液用于体外分析。以每头育肥牛作为试验单位,模型包括谷物加工、添加剂以及谷物加工×添加剂的固定效应。将每个体外培养瓶产生的总气体收集到一个气体收集袋中,用于测定48小时的甲烷浓度。干物质采食量(DMI)未检测到差异(=0.81)。对照或添加剂处理间瘤胃pH值无差异(=0.33)。然而,与干碾压玉米相比,蒸汽压片玉米的瘤胃pH值较低(<0.01)。谷物类型对乙酸与丙酸比例有显著差异(=0.01),添加剂添加有一定趋势(=0.06)。与对照处理相比,添加剂添加降低了总气体中甲烷的比例(≤0.01)。总体而言,莫能酸钠降低了甲烷浓度,不过来源对干物质采食量或瘤胃pH值没有影响。