Crop Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang, 110161, LN, China.
Department of Agricultural and Environmental Sciences, Tennessee State University, Nashville, TN, 37209, USA.
Theor Appl Genet. 2021 Aug;134(8):2379-2398. doi: 10.1007/s00122-021-03830-1. Epub 2021 Jun 14.
Methyl esterase (MES), PvMES1, contributes to the defense response toward Fusarium wilt in common beans by regulating the salicylic acid (SA) mediated signaling pathway from phenylpropanoid synthesis and sugar metabolism as well as others. Common bean (Phaseolus vulgaris L.) is an important food legume. Fusarium wilt caused by Fusarium oxysporum f. sp. phaseoli is one of the most serious soil-borne diseases of common bean found throughout the world and affects the yield and quality of the crop. Few sources of Fusarium wilt resistance exist in legumes and most are of quantitative inheritance. In this study, we have identified a methyl esterase (MES), PvMES1, that contributes to plant defense response by regulating the salicylic acid (SA) mediated signaling pathway in response to Fusarium wilt in common beans. The result showed the role of PvMES1 in regulating SA levels in common bean and thus the SA signaling pathway and defense response mechanism in the plant. Overexpression of the PvMES1 gene enhanced Fusarium wilt resistance; while silencing of the gene caused susceptibility to the diseases. RNA-seq analysis with these transiently modified plants showed that genes related to SA level changes included the following gene ontologies: (a) phenylpropanoid synthesis; (b) sugar metabolism; and (c) interaction between host and pathogen as well as others. These key signal elements activated the defense response pathway in common bean to Fusarium wilt. Collectively, our findings indicate that PvMES1 plays a pivotal role in regulating SA biosynthesis and signaling, and increasing Fusarium wilt resistance in common bean, thus providing novel insight into the practical applications of both SA and MES genes and pathways they contribute to for developing elite crop varieties with enhanced broad-spectrum resistance to this critical disease.
甲酯酶（MES），PvMES1，通过调节苯丙烷合成和糖代谢以及其他途径的水杨酸（SA）介导的信号通路，参与菜豆对枯萎病的防御反应。菜豆（Phaseolus vulgaris L.）是一种重要的食用豆类。由尖孢镰刀菌 f. sp. phaseoli 引起的枯萎病是世界各地普遍存在的最严重的土传病害之一，影响作物的产量和品质。豆类中很少有枯萎病抗性来源，而且大多数都是数量遗传的。在这项研究中，我们鉴定了一种甲酯酶（MES），PvMES1，它通过调节水杨酸（SA）介导的信号通路来参与菜豆对枯萎病的防御反应。结果表明，PvMES1 在调节菜豆中 SA 水平方面发挥作用，从而调节植物中的 SA 信号通路和防御反应机制。过表达 PvMES1 基因增强了对枯萎病的抗性；而基因沉默则导致对疾病的易感性。对这些瞬时修饰植物进行 RNA-seq 分析表明，与 SA 水平变化相关的基因包括以下基因本体论：（a）苯丙烷合成；（b）糖代谢；（c）宿主与病原体的相互作用以及其他。这些关键信号元件激活了菜豆对枯萎病的防御反应途径。总之，我们的研究结果表明，PvMES1 在调节 SA 生物合成和信号转导以及提高菜豆对枯萎病的抗性方面发挥着关键作用，从而为 SA 和 MES 基因及其参与的信号通路在开发具有广谱抗性的优良作物品种方面的实际应用提供了新的见解。
