Wang Chen, Ahsan Taswar, Ding Ao, Han Di, Gao Jie, Liang Chun-Hao, Du Si-Tong, Wei Yi, Huang Yu-Qian, Zhang Shi-Hong
The Key Laboratory for Extreme-Environmental Microbiology, College of Plant Protection, Shenyang Agricultural University, Shenyang, 110866, China.
Institute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang, 110161, China.
World J Microbiol Biotechnol. 2025 Jan 10;41(2):28. doi: 10.1007/s11274-024-04244-z.
The endophytic fungus Serendipita indica (Si) could suppress Phoma arachidicola (Pa) and control peanut web blotch disease. The study evaluated its growth-promoting and disease-resistant effects in two peanut cultivars, Luhua11 and Baisha1016. In vitro experiments and microscopy analysis demonstrated that S. indica suppressed the growth of P. arachidicola. Additionally, scanning electron microscopy illustrated that S. indica adversely affected the pathogen's hyphae. LSi treatment showed the highest stem height (35 cm), root length (15.533 cm), shoot fresh weight (9.33 g), shoot dry weight (1.30085 g), root dry weight (0.1990 g), and chlorophyll a (1.3253) and b (1.8316), while BPa had the lowest values of these parameters. The highest MDA value was observed at 96 h for BPa with (3.14598 nmol/g), and the highest proline value was observed at 72 h for LSi-Pa with (56.42851 µmol/g). Antioxidant enzymes, catalase, peroxidase, ascorbate peroxidase, and phenylalanine ammonia-lyase, increased significantly after 48 h in cultivar L. The most significant result is observed in salicylic acid with LSi-Pa at 72 h (702.10 µg/mL), showing a consistent significant difference. RNA-seq analysis revealed more pronounced transcriptomic changes in cultivar L, with enriched pathways related to flavonoid biosynthesis and defense responses. The LSi-Pa treatment significantly upregulated gene expression at 96 h, with AhNPR1 (0.05807), AhNPR10 (0.10536), AhPAL1 (4.30831), and Ahcapx (0.22074), demonstrating a strong regulatory effect. These results demonstrate that S. indica enhances peanut plant growth and resilience against P. arachidicola, mainly through modulation of oxidative stress and immune responses.
内生真菌印度被孢霉（Si）能够抑制落花生茎点霉（Pa）并防治花生网斑病。本研究评估了其在两个花生品种鲁花11号和白沙1016中的促生长和抗病作用。体外实验和显微镜分析表明，印度被孢霉抑制了落花生茎点霉的生长。此外，扫描电子显微镜显示印度被孢霉对病原菌的菌丝有不利影响。LSi处理的茎高最高（35厘米）、根长最长（15.533厘米）、地上部鲜重最重（9.33克）、地上部干重最重（1.30085克）、根干重最重（0.1990克），叶绿素a（1.3253）和叶绿素b（1.8316）含量最高，而BPa处理的这些参数值最低。BPa处理在96小时时丙二醛（MDA）值最高（3.14598纳摩尔/克），LSi-Pa处理在72小时时脯氨酸值最高（56.42851微摩尔/克）。在品种L中，48小时后过氧化氢酶、过氧化物酶、抗坏血酸过氧化物酶和苯丙氨酸解氨酶等抗氧化酶显著增加。在72小时时，水杨酸与LSi-Pa处理的结果最为显著（702.10微克/毫升），呈现出一致的显著差异。RNA测序分析显示，品种L中的转录组变化更为明显，与类黄酮生物合成和防御反应相关的途径富集。LSi-Pa处理在96小时时显著上调基因表达，其中AhNPR1（0.05807）、AhNPR10（0.10536）、AhPAL1（4.30831）和Ahcapx（0.22074），显示出强烈的调控作用。这些结果表明，印度被孢霉主要通过调节氧化应激和免疫反应来增强花生植株的生长和对落花生茎点霉的抗性。
