CQM‒Centro de Química da Madeira, MMRG, Universidade da Madeira, Campus Universitário da Penteada, 9020-105 Funchal, Portugal.
Faculty of Polytechnics, University of Donja Gorica, Oktoih 1, 81000 Podgorica, Montenegro.
J Zhejiang Univ Sci B. 2021 Jun 15;22(6):450-461. doi: 10.1631/jzus.B2000683.
In this study, the fibers of invasive species L. and L. were successfully used for the first time as new sources to produce cytocompatible and highly crystalline cellulose nanofibers. Cellulose nanofibers were obtained by two methods, based on either alkaline or acid hydrolysis. The morphology, chemical composition, and crystallinity of the obtained materials were characterized by scanning electron microscopy (SEM) together with energy-dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy. The crystallinity indexes (CIs) of the cellulose nanofibers extracted from and were very high (94.1% and 92.7%, respectively). Biological studies evaluating the cytotoxic effects of the prepared cellulose nanofibers on human embryonic kidney 293T (HEK293T) cells were also performed. The nanofibers obtained using the two different extraction methods were all shown to be cytocompatible in the concentration range assayed (i.e., 0‒500 µg/mL). Our results showed that the nanocellulose extracted from and fibers has high potential as a new renewable green source of highly crystalline cellulose-based cytocompatible nanomaterials for biomedical applications.
在这项研究中,首次成功地将入侵物种 L. 和 L. 的纤维用作新的来源,以生产细胞相容性和高结晶度的纤维素纳米纤维。通过两种方法,基于碱性或酸性水解,获得了纤维素纳米纤维。通过扫描电子显微镜 (SEM) 结合能量色散 X 射线光谱 (EDX)、动态光散射 (DLS)、X 射线衍射 (XRD) 和傅里叶变换红外 (FTIR) 光谱对获得的材料的形貌、化学组成和结晶度进行了表征。从 和 中提取的纤维素纳米纤维的结晶度指数 (CI) 非常高(分别为 94.1%和 92.7%)。还进行了评估制备的纤维素纳米纤维对人胚肾 293T(HEK293T)细胞的细胞毒性影响的生物学研究。用两种不同的提取方法获得的纳米纤维在测定的浓度范围内(即 0‒500μg/ml)均表现出细胞相容性。我们的结果表明,从 和 纤维中提取的纳米纤维素具有作为用于生物医学应用的基于纤维素的高结晶度细胞相容性纳米材料的可再生绿色新来源的巨大潜力。