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II类内含子RNA在体外对隐蔽5'剪接位点的选择

Selection of cryptic 5' splice sites by group II intron RNAs in vitro.

作者信息

Müller M W, Schweyen R J, Schmelzer C

机构信息

Institut für Genetik und Mikrobiologie, Universität München, FRG.

出版信息

Nucleic Acids Res. 1988 Aug 11;16(15):7383-95. doi: 10.1093/nar/16.15.7383.

DOI:10.1093/nar/16.15.7383
PMID:3412889
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC338415/
Abstract

Recognition of 5' splice points by group I and group II self-splicing introns involves the interaction of exon sequences--directly preceding the 5' splice site--with intronic sequence elements. We show here that the exon binding sequences (EBS) of group II intron aI5c can accept various substitutes of the authentic intron binding sites (IBS) provided in cis or in trans. The efficiency of cleavages at these cryptic 5' splice sites was enhanced by deletion of the authentic IBS2 element. All cryptic 5' cleavage sites studied here were preceded by an IBS1 like sequence; indicating that the IBS1/EBS1 pairing alone is sufficient for proper 5' splice site selection by the intronic EBS element. The results are discussed in terms of minimal requirements for 5' cleavages and position effects of IBS sites relative to the intron.

摘要

I 组和 II 组自我剪接内含子对 5' 剪接位点的识别涉及外显子序列（恰好在 5' 剪接位点之前）与内含子序列元件的相互作用。我们在此表明，II 组内含子 aI5c 的外显子结合序列（EBS）可以接受顺式或反式提供的真实内含子结合位点（IBS）的各种替代物。通过缺失真实的 IBS2 元件，这些隐蔽 5' 剪接位点处的切割效率得以提高。此处研究的所有隐蔽 5' 切割位点之前都有一个类似 IBS1 的序列；这表明单独的 IBS1/EBS1 配对足以让内含子 EBS 元件进行正确的 5' 剪接位点选择。我们根据 5' 切割的最低要求以及 IBS 位点相对于内含子的位置效应来讨论这些结果。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/651b/338415/e32ddf36e8e7/nar00157-0176-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/651b/338415/6ae84d841f9b/nar00157-0171-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/651b/338415/9f0052545c5d/nar00157-0172-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/651b/338415/e5596967c3d7/nar00157-0173-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/651b/338415/bb731f6221c1/nar00157-0175-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/651b/338415/e32ddf36e8e7/nar00157-0176-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/651b/338415/6ae84d841f9b/nar00157-0171-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/651b/338415/9f0052545c5d/nar00157-0172-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/651b/338415/e5596967c3d7/nar00157-0173-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/651b/338415/bb731f6221c1/nar00157-0175-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/651b/338415/e32ddf36e8e7/nar00157-0176-a.jpg

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DNA polymerization catalysed by a group II intron RNA in vitro.体外由II组内含子RNA催化的DNA聚合反应。

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