Department of Veterinary Medicine and Animal Production, University of Napoli Federico II, Napoli, Italy.
Marine Turtle Research Center, Stazione Zoologica Anton Dohrn, 80055 Portici, Italy.
J Zoo Wildl Med. 2021 Jun;52(2):604-609. doi: 10.1638/2020-0070.
This study aimed to evaluate intraocular pressure (IOP) estimates in healthy eyes of using rebound tonometry in comparison with applanation tonometry. Twenty-three healthy (housed at the Marine Turtle Research Center) without preexisting ophthalmic disease were enrolled in the study. IOP measurements were obtained by the same ophthalmologist, with the turtle in ventral recumbency between 2:30 p.m. and 4:30 p.m., using a rebound tonometer (RT; TonoVet) in dog calibration mode, and after topical anesthesia, an applanation tonometer (AT; Tono-Pen) in both eyes. The average of three readings per instrument was used for analysis. The agreement between the two tonometers was assessed by Bland-Altman analysis and intraclass correlation coefficient (ICC). Moreover, differences in IOP between the two tonometers were analyzed using the Mann-Whitney test. Moderate agreement was found between the two tonometers (ICC, 0.663; 95% confidence interval, 0.206-0.857). The median, Q1, and Q3 IOP obtained with AT (6.2, 4.7, and 9.1 mm Hg) were significantly lower ( = 0.001) than that obtained with RT (9.7, 8.3, and 11.6 mm Hg). It was not possible to obtain an instrument automatically generated mean of four values with AT because of retraction of the globe by the animals, and IOP measurement was unsuccessful in 7 eyes. In conclusion, IOP readings from the RT were statistically higher than those from the AT. RT proved to be more feasible because of the light, short-lasting contact with the cornea.
本研究旨在评估使用回弹眼压计（RT）测量健康海龟眼内压（IOP）的结果，并与压平眼压计（AT）的测量结果进行比较。共纳入 23 只无眼部疾病的健康海龟（居住在海龟研究中心）。由同一名眼科医生在下午 2:30 至 4:30 之间，在海龟腹卧姿势下，使用狗校准模式的 RT（TonoVet）和局部麻醉后，在双眼使用 AT（Tono-Pen）进行IOP 测量。每个仪器测量三次取平均值进行分析。采用 Bland-Altman 分析和组内相关系数（ICC）评估两种眼压计之间的一致性。此外，还使用 Mann-Whitney 检验分析两种眼压计之间的 IOP 差异。两种眼压计之间存在中度一致性（ICC，0.663；95%置信区间，0.206-0.857）。AT 测量的 IOP 中位数、Q1 和 Q3 值（6.2、4.7 和 9.1mmHg）显著低于 RT（9.7、8.3 和 11.6mmHg）（ = 0.001）。由于动物眼球回缩，无法使用 AT 自动生成四个值的平均值，且 7 只眼的眼压测量失败。总之，RT 测量的IOP 值明显高于 AT。由于与角膜的接触轻且持续时间短，RT 更具可行性。
