Department of Bioengineering and Therapeutic Sciences, University of California at San Francisico, San Francisco, California 94158, USA.
Program in Human Genetics, University of California at San Francisco, San Francisco, California 94158, USA.
Learn Mem. 2021 Jun 15;28(7):228-238. doi: 10.1101/lm.053425.121. Print 2021 Jul.
Balancing exploration and anti-predation are fundamental to the fitness and survival of all animal species from early life stages. How these basic survival instincts drive learning remains poorly understood. Here, using a light/dark preference paradigm with well-controlled luminance history and constant visual surrounding in larval zebrafish, we analyzed intra- and intertrial dynamics for two behavioral components, dark avoidance and center avoidance. We uncover that larval zebrafish display short-term learning of dark avoidance with initial sensitization followed by habituation; they also exhibit long-term learning that is sensitive to trial interval length. We further show that such stereotyped learning patterns is stimulus-specific, as they are not observed for center avoidance. Finally, we demonstrate at individual levels that long-term learning is under homeostatic control. Together, our work has established a novel paradigm to understand learning, uncovered sequential sensitization and habituation, and demonstrated stimulus specificity, individuality, as well as dynamicity in learning.
从生命早期阶段起,平衡探索和反捕食对于所有动物物种的适应性和生存都是至关重要的。这些基本生存本能如何驱动学习仍然知之甚少。在这里,我们使用具有良好控制的亮度历史和恒定视觉环境的亮/暗偏好范式,分析了幼虫斑马鱼的两个行为成分(避光和中心回避)的内和试验间动力学。我们发现,幼虫斑马鱼表现出对避光的短期学习,最初是敏感化,随后是习惯化;它们还表现出对试验间隔长度敏感的长期学习。我们进一步表明,这种刻板的学习模式是刺激特异性的,因为它们在中心回避中观察不到。最后,我们在个体水平上证明了长期学习受到体内平衡控制。总的来说,我们的工作建立了一个理解学习的新范式,揭示了顺序敏感化和习惯化,并证明了学习的刺激特异性、个体性和动态性。