Blackburn E H, Challoner P B
Cell. 1984 Feb;36(2):447-57. doi: 10.1016/0092-8674(84)90238-1.
A simple repetitive DNA sequence in the nuclear genome of Trypanosoma brucei, consisting of tandem repeats of the hexanucleotide 5' CCCTAA 3', was identified as being telomeric by several criteria. This sequence was specifically labeled with T. brucei genomic DNA as the template for in vitro nick translation by DNA polymerase I, and was present in Bal 31 nuclease sensitive, genomic restriction fragments of the large sizes expected in this organism for at least some telomeric regions. The same repeated sequence was found in six other flagellates tested. A segment of DNA from T. brucei including this telomeric sequence was cloned in pBR322 and characterized. The cloned segment contained a sequence highly homologous to the 3' ends of several variant surface glycoprotein mRNAs, upstream of the tandemly repeated hexanucleotide sequence.
布氏锥虫核基因组中的一个简单重复DNA序列，由六核苷酸5' CCCTAA 3'的串联重复组成，通过几个标准被鉴定为端粒序列。该序列以布氏锥虫基因组DNA为模板，经DNA聚合酶I进行体外切口平移而被特异性标记，并且存在于Bal 31核酸酶敏感的基因组限制性片段中，这些片段是该生物体至少某些端粒区域预期的大尺寸片段。在测试的其他六种鞭毛虫中也发现了相同的重复序列。来自布氏锥虫的一段包含该端粒序列的DNA被克隆到pBR322中并进行了表征。克隆片段在串联重复的六核苷酸序列上游包含一个与几种可变表面糖蛋白mRNA的3'端高度同源的序列。
