Landegren U, Kaiser R, Sanders J, Hood L
Division of Biology, California Institute of Technology, Pasadena 91125.
Science. 1988 Aug 26;241(4869):1077-80. doi: 10.1126/science.3413476.
An assay for the presence of given DNA sequences has been developed, based on the ability of two oligonucleotides to anneal immediately adjacent to each other on a complementary target DNA molecule. The two oligonucleotides are then joined covalently by the action of a DNA ligase, provided that the nucleotides at the junction are correctly base-paired. Thus single nucleotide substitutions can be distinguished. This strategy permits the rapid and standardized identification of single-copy gene sequences in genomic DNA.
基于两种寡核苷酸在互补靶DNA分子上彼此紧邻退火的能力，已开发出一种检测给定DNA序列存在的方法。然后，只要连接处的核苷酸正确碱基配对，这两种寡核苷酸就会在DNA连接酶的作用下共价连接。因此，可以区分单核苷酸替换。该策略允许快速、标准化地鉴定基因组DNA中的单拷贝基因序列。
