Junita Dila, Prasetyo Agung Aji, Muniroh Muflihatul, Kristina Tri Nur, Mahati Endang
General Surgery Department, Diponegoro University / Dr. Kariadi Central Hospital Semarang, 50244, Indonesia.
Pediatric Surgery Department, Diponegoro University / Dr. Kariadi Central Hospital Semarang, 50244, Indonesia.
Ann Med Surg (Lond). 2021 May 25;66:102406. doi: 10.1016/j.amsu.2021.102406. eCollection 2021 Jun.
Peritonitis is the second most common cause of severe sepsis that associated with a significant mortality rate. Due to a large gap of newer antibiotics innovation and antibiotic resistance emergence, the use of antioxidant has a possible alternative as adjuvant therapy in peritonitis management. It has been studied that glutathione as an alternative in the development of new anti-inflammatory effect. Thus, the aim of this study was to evaluate the levels of TNF-α and IL-10 after glutathione administration as adjuvant therapy in rat peritonitis model.
Male wistar rats were divided into four groups (n = 6 per group), Group 1: control group (C), Group 2: peritonitis group (P), Group 3: peritonitis + Ceftriaxone group (P + Cef), Group 4: peritonitis + Ceftriaxone + Glutathione group (P + Cef + Glu). Twenty-four hours after peritonitis induction, the blood samples were taken to evaluate TNF-α and IL-10 levels.
There was a significantly increase of mean TNF-α level in group 2 (P) 473,86 ± 388,99 pg/ml (p value 0,00) and significantly decrease of mean TNF-α level after glutathione injection in group 4 (P + Cef + Glu) (p value 0,02). No significant changes in IL-10 levels in rats peritonitis model.
Glutathione supplementation is significantly decrease the mean level of TNF-α in rats induced peritonitis, however there is no difference compare to antibiotic only. Moreover, there no significant changes level of IL-10 in rats induced peritonitis after glutathione injection.
腹膜炎是严重脓毒症的第二大常见病因，其死亡率颇高。由于新型抗生素研发与抗生素耐药性出现之间存在巨大差距，抗氧化剂有可能作为腹膜炎治疗中的辅助疗法。已有研究表明谷胱甘肽可作为产生新抗炎作用的替代物。因此，本研究旨在评估在大鼠腹膜炎模型中，谷胱甘肽作为辅助疗法给药后肿瘤坏死因子-α（TNF-α）和白细胞介素-10（IL-10）的水平。
雄性Wistar大鼠分为四组（每组n = 6），第1组：对照组（C），第2组：腹膜炎组（P），第3组：腹膜炎 + 头孢曲松组（P + Cef），第4组：腹膜炎 + 头孢曲松 + 谷胱甘肽组（P + Cef + Glu）。诱导腹膜炎24小时后，采集血样以评估TNF-α和IL-10水平。
第2组（P）的平均TNF-α水平显著升高，为473.86 ± 388.99 pg/ml（p值0.00），第4组（P + Cef + Glu）注射谷胱甘肽后平均TNF-α水平显著降低（p值0.02）。大鼠腹膜炎模型中IL-10水平无显著变化。
补充谷胱甘肽可显著降低诱导性腹膜炎大鼠的平均TNF-α水平，但与仅使用抗生素相比无差异。此外，注射谷胱甘肽后诱导性腹膜炎大鼠的IL-10水平无显著变化。
