Ferronatto Andressa Neuhaus, Rossi Rochele, Massochin Nunes Pinto Laura, Garavaglia Juliano
NUTRIFOR Research Institute in Food and Health at UNISINOS University, Unisinos Avenue, 950 - São Leopoldo, Rio Grande do Sul, 93022-750, Brazil.
Biotechnol Rep (Amst). 2021 May 24;30:e00636. doi: 10.1016/j.btre.2021.e00636. eCollection 2021 Jun.
This study aimed to assess the growth potential of and in rice bran, a co-product from the food industry, and subsequently develop a freeze-dried symbiotic. Furthermore, phytochemicals and antioxidant properties were analysed. The growth was measured using growth kinetics over 72 h. The total phenolic compounds were analysed by the Folin-Ciocalteau method and antioxidant potential by DPPH and ABS methods. Freeze-drying process occurred using a pilot-scale equipment (Liotop LP510), verification and quantification of probiotics occurred through molecular analyses, as DNA extraction and PCR. As a result, there was a good growth in rice bran (p = 0.04), suggesting its prebiotic potential. Rice bran also showed significant concentrations of phenolic compounds (3.69 mgEAG/mL ± 0.04) and antioxidant activity according ABTS (8.35 μmol ET/mL ± 0.106) and DPPH (24.71 μmol ET/mL ± 7.90) methods. The bacteria concentration decreased significantly when submitted to the freeze-drying process (p = 0.001), however, they remained by the minimum concentration required for a product to be considered a symbiotic. Therefore, it was concluded that rice bran and these analysed bacteria proved to be effective for a symbiotic formulation.
本研究旨在评估食品工业副产品米糠中[具体菌种未给出]和[具体菌种未给出]的生长潜力，并随后开发一种冻干共生体。此外，还分析了植物化学物质和抗氧化特性。通过72小时的生长动力学来测量生长情况。采用福林-西奥尔特法分析总酚类化合物，采用DPPH法和ABS法分析抗氧化潜力。使用中试规模设备（Liotop LP510）进行冷冻干燥过程，通过分子分析（如DNA提取和PCR）对益生菌进行验证和定量。结果，米糠中出现了良好的生长情况（p = 0.04），表明其具有益生元潜力。根据ABTS法（8.35 μmol ET/mL ± 0.106）和DPPH法（24.71 μmol ET/mL ± 7.90），米糠还显示出显著浓度的酚类化合物（3.69 mgEAG/mL ± 0.04）和抗氧化活性。当进行冷冻干燥过程时，细菌浓度显著下降（p = 0.001），然而，它们仍保持在产品被视为共生体所需的最低浓度以上。因此，得出的结论是，米糠和这些分析的细菌被证明对共生体配方有效。
