Yu Dong, Wang Tengjiao, Liang Dong, Mei Yue, Zou Wenbin, Guo Shiwei
Center of Translational Medicine, Second Military Medical University, Shanghai, China.
Shanghai Key Laboratory of Cell Engineering, Shanghai, China.
Front Oncol. 2021 May 31;11:651350. doi: 10.3389/fonc.2021.651350. eCollection 2021.
Recent research studies on interrogation of the tumor microbiome (including bacteria, viruses, and fungi) have yielded important insights into the role of microbes in carcinogenesis, therapeutic responses, and resistance. Once thought to be a sterile organ, a number of studies have showed the presence of microbes within this organ in PDAC status. A microbiome-pancreas axis for PDAC (pancreatic ductal adenocarcinoma) carcinogenesis is proposed. However, the microbial composition of localized PDAC tissue is still unclear. The associations between microbiome and PDAC reported in previous studies were detected in an indirect way, which mostly used samples from stool, oral saliva, and intestinal samples. This study integrated 582 samples derived from PDAC tissues across four datasets and presented a landscape of tumor microbiome at the genus level in PDAC based on remining of RNA-Seq data. On average, there are hundreds of genera distributed in the PDAC tissue, and dozens of core microbiota were identified by PDAC tissue. The pan-microbiome of PDAC tissue was also estimated, which might surpass 2,500 genera. In addition, sampling sites (stroma . epithelium) and tissue source (human tissue . PDX) were found to have great effects on the microbial composition of PDAC tissue, but not the traditional risk factors (sex and age). It is the first study to systematically focus on exploring the microbial composition of PDAC tissue and is helpful to have a deep understanding of tumor microbiome. The identified specific taxa might be potential biomarkers for follow-up research studies.
近期关于肿瘤微生物组（包括细菌、病毒和真菌）的研究，已就微生物在致癌作用、治疗反应及耐药性方面的作用取得了重要见解。肿瘤曾被认为是一个无菌器官，但多项研究表明，在胰腺癌状态下该器官内存在微生物。有人提出了一条胰腺癌（胰腺导管腺癌）致癌的微生物组 - 胰腺轴。然而，局部胰腺癌组织的微生物组成仍不清楚。以往研究中报道的微生物组与胰腺癌之间的关联是通过间接方式检测到的，大多使用粪便、口腔唾液和肠道样本。本研究整合了来自四个数据集的582份胰腺癌组织样本，并基于RNA测序数据的挖掘展示了胰腺癌在属水平上的肿瘤微生物组概况。平均而言，胰腺癌组织中分布着数百个属，通过胰腺癌组织鉴定出了数十种核心微生物群。还估算出胰腺癌组织的泛微生物组可能超过2500个属。此外，发现采样部位（基质.上皮）和组织来源（人体组织.PDX）对胰腺癌组织的微生物组成有很大影响，但传统风险因素（性别和年龄）则不然。这是第一项系统聚焦于探索胰腺癌组织微生物组成的研究，有助于深入了解肿瘤微生物组。所鉴定出的特定分类群可能是后续研究的潜在生物标志物。
