ALV-miRNA-p19-01 通过靶向双重特异性磷酸酶 6 促进病毒复制。

ALV-miRNA-p19-01 Promotes Viral Replication via Targeting Dual Specificity Phosphatase 6.

机构信息

Guangdong Laboratory of Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.

Guangdong Haid Institute of Animal Husbandry & Veterinary, Guangzhou 511400, China.

出版信息

Viruses. 2022 Apr 13;14(4):805. doi: 10.3390/v14040805.

DOI:10.3390/v14040805

PMID:35458535

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9024826/

Abstract

MicroRNAs (miRNAs) are a group of regulatory noncoding RNAs, serving as major regulators with a sequence-specific manner in multifarious biological processes. Although a series of viral families have been proved to encode miRNAs, few reports were available regarding the function of ALV-J-encoded miRNA. Here, we reported a novel miRNA (designated ALV-miRNA-p19-01) in ALV-J-infected DF-1 cells. We found that ALV-miRNA-p19-01 is encoded by the genome of the ALV-J SCAU1903 strain (located at nucleotides site 779 to 801) in a classic miRNA biogenesis manner. The transfection of DF-1 cells with ALV-miRNA-p19-01 enhanced ALV-J replication, while the blockage of ALV-miRNA-p19-01 suppressed ALV-J replication. Furthermore, our data showed that ALV-miRNA-p19-01 promotes ALV-J replication by directly targeting the cellular gene dual specificity phosphatase 6 through regulating ERK2 activity.

摘要

微小 RNA（miRNAs）是一组调控性非编码 RNA，作为主要的调控因子，以序列特异性方式参与多种生物学过程。虽然已经证实一系列病毒家族编码 miRNA，但关于 ALV-J 编码 miRNA 的功能的报道却很少。在这里，我们在感染 ALV-J 的 DF-1 细胞中报告了一种新型 miRNA（命名为 ALV-miRNA-p19-01）。我们发现，ALV-miRNA-p19-01 是通过经典 miRNA 生物发生方式由 ALV-J SCAU1903 株（位于核苷酸位置 779 到 801）的基因组编码的。用 ALV-miRNA-p19-01 转染 DF-1 细胞可增强 ALV-J 的复制，而阻断 ALV-miRNA-p19-01 则抑制 ALV-J 的复制。此外，我们的数据表明，ALV-miRNA-p19-01 通过调节 ERK2 活性直接靶向细胞基因双特异性磷酸酶 6，从而促进 ALV-J 的复制。