Simon K, Lingappa V R, Ganem D
Department of Microbiology, University of California Medical Center, San Francisco 94143.
Virology. 1988 Sep;166(1):76-81. doi: 10.1016/0042-6822(88)90148-1.
Hepatitis B surface antigen is the major protein of the virion envelope, and is also independently secreted from infected cells as a subviral particle composed exclusively of HBsAg and host-derived lipid. Similar particles are efficiently assembled and secreted by cultured mammalian cells transfected with the gene for HBsAg. In contrast to such cultured cells, Xenopus oocytes microinjected with HBsAg mRNA secrete less than 5% of newly synthesized HBsAg polypeptides. We have examined the HBsAg biosynthetic intermediates in such oocytes and provide evidence that the impaired secretion of HBsAg is due to a discrete block in the assembly of lipoprotein particles.
乙型肝炎表面抗原是病毒粒子包膜的主要蛋白质，也作为仅由乙肝表面抗原和宿主来源脂质组成的亚病毒颗粒从受感染细胞中独立分泌。用乙肝表面抗原基因转染的培养哺乳动物细胞能高效组装并分泌类似颗粒。与这种培养细胞不同，显微注射乙肝表面抗原信使核糖核酸的非洲爪蟾卵母细胞分泌的新合成乙肝表面抗原多肽不到5%。我们已研究了此类卵母细胞中乙肝表面抗原的生物合成中间体，并提供证据表明乙肝表面抗原分泌受损是由于脂蛋白颗粒组装过程中的一个离散阻滞。
