Department of Pediatric Dentistry and Orthodontics, College of Dentistry, King Saud University, Riyadh, Saudi Arabia.
Division of Orthodontics, Department of Pediatric Dentistry and Orthodontics, College of Dentistry, King Saud University, Riyadh, Saudi Arabia.
Arch Oral Biol. 2020 Nov;119:104883. doi: 10.1016/j.archoralbio.2020.104883. Epub 2020 Sep 12.
This study aimed to investigate the effect of the low intensity pulsed ultrasound (LIPUS) on the dentoalveolar structures during orthodontic force application in ex-vivo model using mandible slice organ culture (MSOC) of diabetic rats.
18 male Wistar rats with a mean weight (275 g) were randomly divided into three main groups: 1) normal rats, 2) Insulin treated diabetic rats, and 3) diabetic rats. Diabetes mellitus (DM) was induced by streptozotocin. Four weeks later, rats were euthanized, mandibles were dissected, divided into 1.5-mm slices creating mandible slice organ cultures (MSOCs). MSOCs were cultured at 37 °C in air with 5 % CO. The following day, orthodontic spring delivering a 50-g of force was applied to each slice. In each group, rats were randomly assigned to 2 subgroups; one received 10 min of LIPUS daily and the other was the control. Culture continued for 7 days, and then the sections were prepared for histological and histomorphometric analysis.
For all study groups (Normal, Insulin Treated Diabetic and Diabetic), LIPUS treatment significantly increased the thickness of predentin, cementum, and improved bone remodeling on the tension side and increased odontoblast, sub-odontoblast, and periodontal ligaments cell counts and bone resorption lacunae number on the compression side.
Application of LIPUS treatment for 10 min daily for a week enhanced bone remodeling and repair of cementum and dentin in normal as well as diabetic MSOCs.
本研究旨在通过糖尿病大鼠下颌骨切片器官培养（MSOC）的离体模型，探讨低强度脉冲超声（LIPUS）在正畸力应用过程中对牙牙槽结构的影响。
18 只雄性 Wistar 大鼠，平均体重（275 克），随机分为三组：1）正常大鼠组；2）胰岛素治疗糖尿病大鼠组；3）糖尿病大鼠组。糖尿病（DM）通过链脲佐菌素诱导。4 周后，处死大鼠，分离下颌骨，切成 1.5 毫米的薄片，制作下颌骨切片器官培养（MSOC）。MSOC 在 37°C 空气中以 5%CO2 培养。次日，在每个切片上施加 50g 的正畸弹簧力。在每组中，将大鼠随机分为 2 个亚组；一组每天接受 10 分钟的 LIPUS 治疗，另一组为对照组。培养继续进行 7 天，然后对切片进行组织学和组织形态计量学分析。
对于所有研究组（正常、胰岛素治疗糖尿病和糖尿病），LIPUS 治疗显著增加了前期牙本质、牙骨质的厚度，并改善了张力侧的骨重塑，增加了成牙本质细胞、亚成牙本质细胞和牙周膜细胞计数以及压缩侧的骨吸收陷窝数。
LIPUS 治疗每天 10 分钟，持续 1 周，可增强正常和糖尿病 MSOC 中的骨重塑和牙骨质及牙本质的修复。
