Student Research Committee, Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Medical Nano-Technology and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Dermatol Ther. 2021 Jul;34(4):e15028. doi: 10.1111/dth.15028. Epub 2021 Jun 28.
Dermal fibroblasts are the most accessible cells in the skin that have gained significant attention in cell therapy. Applying dermal fibroblasts' regenerative capacity can introduce new patterns to develop cell-based therapies to treat skin disorders. Dermal fibroblasts originate from mesenchymal cells and are located within the dermis. These cells are mainly responsible for synthesizing glycosaminoglycans, collagens, and components of extracellular matrix supporting skin's structural integrity. Preclinical studies suggested that allogeneic and autologous dermal fibroblasts provide widespread and beneficial applications for wound healing, burn ulcers, and inherited skin disorders. In this regard, generating induced pluripotent stem cells (iPSCs) from fibroblasts and gene-edited fibroblasts are promising approaches for treating skin disorders. Here, we aimed to review literature about ongoing and completed clinical trials that applied fibroblasts and bioengineered fibroblasts as therapeutic agents for various skin disorders. This review explores cell therapy protocols from the earliest phase of allogeneic and autologous fibroblasts development in different benches to translating them into bedside-level treatment for skin disorders, particularly recessive dystrophic epidermolysis bullosa.
皮肤成纤维细胞是皮肤中最容易获取的细胞，在细胞治疗中受到了广泛关注。利用皮肤成纤维细胞的再生能力，可以为开发基于细胞的治疗方法来治疗皮肤疾病引入新的模式。皮肤成纤维细胞起源于间充质细胞，位于真皮层内。这些细胞主要负责合成糖胺聚糖、胶原和细胞外基质的成分，支持皮肤的结构完整性。临床前研究表明，同种异体和自体皮肤成纤维细胞为创伤愈合、烧伤溃疡和遗传性皮肤疾病提供了广泛而有益的应用。在这方面，从成纤维细胞和基因编辑成纤维细胞中生成诱导多能干细胞（iPSCs）是治疗皮肤疾病的有前途的方法。在这里，我们旨在回顾关于正在进行和已完成的临床试验的文献，这些试验将成纤维细胞和生物工程成纤维细胞作为治疗各种皮肤疾病的治疗剂。本综述探讨了从不同平台的同种异体和自体成纤维细胞开发的早期阶段到将其转化为皮肤疾病（特别是隐性营养不良性大疱性表皮松解症）床边治疗的细胞治疗方案。
