Collaborative Innovation Centre for Green Chemical Manufacturing and Accurate Detection School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, Shandong, China; Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, Shandong, China.
Collaborative Innovation Centre for Green Chemical Manufacturing and Accurate Detection School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, Shandong, China; Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, Shandong, China.
Biosens Bioelectron. 2021 Nov 1;191:113409. doi: 10.1016/j.bios.2021.113409. Epub 2021 Jun 11.
In this paper, we report a novel multiple amplification strategy for ultrasensitive near-infrared electrochemiluminescence (ECL) immunoassay in KSO solution. The realization of this strategy is based on the antenna effect of Eu-MOF (EuBTC) and a high efficiency catalysis of CoS hollow triple shelled nanoboxes (TSNBs). The HBTC ligand in the antenna effect first undergoes π-π* absorption and a singlet-singlet electronic transition. Its energy passes through the intersystem to the triplet state, next transfers from the lowest excited triplet state to the vibrational energy level of the rare earth ion, finally realizing sensitizing center ion luminescence. Moreover, ionic reaction and structural advantages endow CoS TSNBs a dual signal enhancement effect. This sandwich-type ECL biosensor has a near-infrared luminescence in 800-900 nm, thus avoiding damage to the sample in the meantime. In practical diagnosis, the normal critical value of procalcitonin (PCT) (<0.5 ng/mL) is much higher than the detection limit (3.65 fg/mL) and is in the detection range (10 fg/mL-100 ng/mL), which means that the ECL biosensor has a high sensitivity in the detection of PCT and meet the requirement for diagnosis of disease completely. Therefore, the strategy provides a feasible method for efficient and stable analysis of systemic inflammatory response such as fearful bacterial infection, hepatitis B, and peritonitis.
在本文中，我们报告了一种在 KSO 溶液中用于超灵敏近红外电化学发光（ECL）免疫分析的新型多重扩增策略。该策略的实现基于 Eu-MOF（EuBTC）的天线效应和 CoS 空心三重壳纳米盒（TSNBs）的高效催化作用。天线效应中的 HBTC 配体首先经历π-π*吸收和单重-单重电子跃迁。其能量通过系间穿越到三重态，然后从最低激发三重态转移到稀土离子的振动能级，最终实现敏化中心离子发光。此外，离子反应和结构优势赋予了 CoS TSNBs 双重信号增强效应。这种三明治型 ECL 生物传感器在 800-900nm 处具有近红外发光，同时避免了对样品的损伤。在实际诊断中，降钙素原（PCT）的正常临界值（<0.5ng/mL）远高于检测限（3.65fg/mL）且处于检测范围（10fg/mL-100ng/mL）内，这意味着 ECL 生物传感器在 PCT 的检测中具有很高的灵敏度，完全满足疾病诊断的要求。因此，该策略为高效稳定地分析全身性炎症反应（如可怕的细菌感染、乙型肝炎和腹膜炎）提供了一种可行的方法。
