Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St. Lucia, Queensland, Australia.
Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St. Lucia, Queensland, Australia.
Colloids Surf B Biointerfaces. 2021 Oct;206:111923. doi: 10.1016/j.colsurfb.2021.111923. Epub 2021 Jun 15.
Labeling nanoparticles with fluorescent dyes is a common approach to investigate their cell uptake and biodistribution, providing valuable information for the preclinical assessment of nanoparticles for drug delivery. However, the underlying assumption that the fluorescence intensity of dye-labeled nanoparticles correlates positively with the amount of nanoparticles taken up by cells might not be valid under some conditions, as it can be affected by many factors including dye dispersion, dye quenching, and material shading. Here we demonstrated that both nanoparticles with hydrophobic dyes encapsulated inside and nanoparticles with hydrophilic dyes conjugated on the particle surface suffer from different degrees of dye quenching, making it challenging for quantitative comparison of cell uptake of different nanoparticles. To address this challenge, we proposed a possible solution for direct comparative studies of dye-labeled nanoparticles. This work provides valuable information for designing and evaluating different nanoparticles for drug delivery applications.
用荧光染料标记纳米颗粒是研究其细胞摄取和生物分布的常用方法，为纳米颗粒用于药物输送的临床前评估提供了有价值的信息。然而，荧光强度与细胞摄取的纳米颗粒数量呈正相关的假设在某些情况下可能并不成立，因为它会受到许多因素的影响，包括染料分散、染料猝灭和材料遮蔽。在这里，我们证明了内部封装疏水性染料的纳米颗粒和表面连接亲水性染料的纳米颗粒都受到不同程度的染料猝灭，这使得定量比较不同纳米颗粒的细胞摄取变得具有挑战性。为了解决这个挑战，我们提出了一种用于直接比较染料标记纳米颗粒的可能方法。这项工作为设计和评估用于药物输送应用的不同纳米颗粒提供了有价值的信息。
