Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.
ARC Centre of Excellence for Enabling Eco-Efficient Beneficiation of Minerals, The University of Queensland, Brisbane, QLD 4072, Australia.
Biosensors (Basel). 2021 Dec 9;11(12):505. doi: 10.3390/bios11120505.
Fluorescence labelling is often used for tracking nanoparticles, providing a convenient assay for monitoring nanoparticle drug delivery. However, it is difficult to be quantitative, as many factors affect the fluorescence intensity. Förster resonance energy transfer (FRET), taking advantage of the energy transfer from a donor fluorophore to an acceptor fluorophore, provides a distance ruler to probe NP drug delivery. This article provides a review of different FRET approaches for the ratiometric monitoring of the self-assembly and formation of nanoparticles, their in vivo fate, integrity and drug release. We anticipate that the fundamental understanding gained from these ratiometric studies will offer new insights into the design of new nanoparticles with improved and better-controlled properties.
荧光标记常用于追踪纳米粒子，为监测纳米粒子药物输送提供了一种方便的检测方法。然而，由于许多因素会影响荧光强度，因此很难进行定量分析。荧光共振能量转移（Förster resonance energy transfer，FRET）利用供体荧光团向受体荧光团的能量转移，提供了一个距离标尺来探测 NP 药物输送。本文综述了不同的 FRET 方法，用于比率监测纳米粒子的自组装和形成、它们的体内命运、完整性和药物释放。我们预计，这些比率研究中获得的基本认识将为设计具有改进和更好控制性能的新型纳米粒子提供新的见解。
