Laboratory of Clinical Microbiology, Mycology and Antibiotic Therapy, National Research Center for Hematology, Noviy Zykovskiy proezd 4A, Moscow, 125167, Russian Federation.
Head of the Laboratory of Clinical Microbiology, Mycology and Antibiotic Therapy, National Research Center for Hematology Moscow, Russian Federation.
J Mycol Med. 2021 Sep;31(3):101162. doi: 10.1016/j.mycmed.2021.101162. Epub 2021 Jun 2.
The aim of the present study was to evaluate the in vitro susceptibility of anidulafungin, caspofungin, fluconazole and conventional amphotericin B against biofilms and planktonic forms of Candida species isolated from blood culture in patients with hematological malignancies.
Antifungal susceptibility for planktonic forms and biofilms of Candida was determined by broth microdilution method as described by Clinical and Laboratory Standards Institute M27 methodology and metabolic XTT-based [2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] reduction assay, respectively.
A total of 75 Candida isolates were evaluated between 2006-2018 yy at the National Research Center for Hematology, Russia, Moscow. Biofilm production was detected in 34 (45.3%) Candida species. Antifungal susceptibility was tested for 27 common species of Candida forming biofilms (8 C.krusei, 7 C.tropicalis, 7 C.albicans, 5 C.parapsilosis). MICs below the susceptibility breakpoints were found for 100% of planktonic forms of Candida species for anidulafungin, 85.2% for caspofungin, and 66.7% for fluconazole. Amphotericin B MIC for Candida species were less than or equal to 1 μg/ml. Candida biofilms were susceptible in vitro for both tested echinocandins, but MIC of anidulafungin were lower compared to caspofungin. The highest MIC against Candida biofilms was found for fluconazole (>1,024 μg/ml for all tested isolates) and for conventional amphotericin B (range 4-16 μg/ml).
The majority of Candida isolates grown as planktonic forms were susceptible to anidulafungin, caspofungin, conventional amphotericin B and fluconazole. Anidulafungin displayed higher activity against Candida biofilms than caspofungin. All Candida biofilms were resistant to fluconazole and conventional amphotericin B.
本研究旨在评估安尼鲁单抗、卡泊芬净、氟康唑和常规两性霉素 B 对血液病患者血培养分离的念珠菌生物膜和浮游形式的体外敏感性。
采用临床和实验室标准协会 M27 方法描述的肉汤微量稀释法和基于代谢 XTT 的[2,3-双（2-甲氧基-4-硝基-5-磺苯基）-2H-四唑-5-羧基苯胺]还原测定法分别测定念珠菌浮游形式和生物膜的抗真菌药敏性。
2006-2018 年，俄罗斯莫斯科国家血液学研究中心共评估了 75 株念珠菌分离株。34 株（45.3%）念珠菌检测到生物膜形成。对 27 株形成生物膜的常见念珠菌种（8 株 C.krusei、7 株 C.tropicalis、7 株 C.albicans、5 株 C.parapsilosis）进行了抗真菌药敏试验。安尼鲁单抗对所有念珠菌种浮游形式的 MIC 值均低于药敏折点，为 100%；卡泊芬净为 85.2%；氟康唑为 66.7%。两性霉素 B 的 MIC 值均小于或等于 1μg/ml。两种测试的棘白菌素对念珠菌生物膜均具有体外敏感性，但安尼鲁单抗的 MIC 值低于卡泊芬净。氟康唑对念珠菌生物膜的 MIC 值最高（所有测试分离株均>1024μg/ml），常规两性霉素 B 的 MIC 值范围为 4-16μg/ml。
大多数作为浮游形式生长的念珠菌分离株对安尼鲁单抗、卡泊芬净、常规两性霉素 B 和氟康唑敏感。安尼鲁单抗对念珠菌生物膜的活性高于卡泊芬净。所有念珠菌生物膜均对氟康唑和常规两性霉素 B 耐药。
