Biointerfaces Institute, McMaster University, 1280 Main St W, Hamilton, Ontario, L8S 0A3, Canada.
Department of Chemical Engineering, McMaster University, 1280 Main St W, Hamilton, Ontario, L8S 4L7, Canada.
Sci Rep. 2017 Sep 26;7(1):12335. doi: 10.1038/s41598-017-12549-3.
We present a simple all-in-one paper-based sensor for E. coli detection using a composite ink made of a fluorogenic DNAzyme probe for bacterial recognition and signal generation, lysozyme that lyses whole bacterial cells, and pullulan/trehalose sugars that stabilize printed bioactive molecules. The paper sensor is capable of producing a fluorescence signal as a readout within 5 minutes upon contacting E. coli, can achieve a limit of detection of 100 cells/mL, in a variety of sample matrixes, without sample enrichment, and remains stable for at least 6 months when stored at ambient temperature. Therefore, this simple paper sensor provides rapid bacterial testing on site, and can be shipped and stored under ambient conditions to benefit users living in resource-limited regions.
我们提出了一种简单的基于纸张的传感器，用于使用由荧光 DNA 酶探针组成的复合油墨检测大肠杆菌，该探针用于细菌识别和信号产生，溶菌酶用于裂解完整的细菌细胞，以及普鲁兰/海藻糖糖用于稳定打印的生物活性分子。该纸张传感器在与大肠杆菌接触后 5 分钟内即可产生荧光信号作为读数，可以在无需样品富集的情况下，在各种样品基质中达到 100 个细胞/mL 的检测限，并且在环境温度下储存至少 6 个月时仍然稳定。因此，这种简单的纸张传感器可以提供现场快速细菌检测，并可以在环境条件下运输和储存，以造福生活在资源有限地区的用户。
