Unravelling Phytotoxicity and Mode of Action of Tripyrasulfone, a Novel Herbicide.

Tripyrasulfone is a novel herbicide post-emergence applied in paddy fields. In this study, tripyrasulfone phytotoxicity and its mode of action were investigated. Within 3-7 days after treatment (DAT), tripyrasulfone caused strong bleaching symptoms on newly developed leaves of followed by necrosis prior to death within 14 DAT. By investigating pigment composition, photosynthetic activity and energy dissipation of treated with tripyrasulfone, the accumulation of phytoene and significant decreases in total carotenoids were observed; the photosystem II complex (PSII) reaction center and PSII-PSI electron transport chain were damaged; and the non-photochemical energy quenching and reactive oxygen species were significantly increased. Based on the reversion of bleaching symptoms in treated by the addition of homogentisic acid, it was hypothesized that tripyrasulfone blocks the biosynthesis of HGA, possibly by the inhibition of 4-hydroxyphenylpyruvate dioxygenase (HPPD). However, based on its chemical structure, tripyrasulfone may tend to be hydrolyzed in plants. Indeed, the hydrolyzed tripyrasulfone (HDT) inhibited the activity of HPPD from produced by , which was approximately 6 times less effective than mesotrione. Molecular docking showed that the HDT formed a stable bidentate interaction with the active center Fe chelation of HPPD.