Isolation and Identification of Herbicidal Active Compounds from L. and Exploration of the Binding Sites of Brassicanate A Sulfoxide.

L. has strong allelopathic effects on weeds. However, the allelochemicals with herbicidal activity in L. are still unknown. In this study, we evaluated the activity of allelochemicals isolated from L. based on the germination and growth of model plant Linn., grass weed , and broadleaf weed Additionally, we employed molecular docking to predict the binding of brassicanate A sulfoxide to herbicide targets. The results of this study showed that eight compounds with herbicidal activity were isolated from L., and the predicted results indicated that brassicanate A sulfoxide was stably bound to dihydroxyacid dehydratase, hydroxymethylpyruvate dioxygenase, acetolactate synthase, PYL family proteins and transport inhibitor response 1. This research provides compound sources and a theoretical foundation for the development of natural herbicides.