Bown D, Ellis T H, Gatehouse J A
Department of Botany, University of Durham, U.K.
Biochem J. 1988 May 1;251(3):717-26. doi: 10.1042/bj2510717.
The sequence of a gene encoding convicilin, a seed storage protein in pea (Pisum sativum L.), is reported. This gene, designated cvcA, is one of a sub-family of two active genes. The transcription start of cvcA was mapped. Convicilin genes are expressed in developing pea seed cotyledons, with maximum levels of the corresponding mRNA species present at 16-18 days after flowering. The gene sequence shows that convicilin is similar to vicilin, but differs by the insertion of a 121-amino-acid sequence near the N-terminus of the protein. This inserted sequence is very hydrophilic and has a high proportion of charged and acidic residues; it is of a similar amino acid composition to the sequences found near the C-terminal of the alpha-subunit in pea legumin genes, but is not directly homologous with them. Comparison of this sequence with the 'inserted' sequence in soya-bean (Glycine max) conglycinin (a homologous vicilin-type protein) suggests that the two insertions were independent events. The 5' flanking sequence of the gene contains several putative regulatory elements, besides a consensus promoter sequence.
报道了编码豌豆（Pisum sativum L.）种子贮藏蛋白伴刀豆球蛋白的基因序列。该基因命名为cvcA，是两个活性基因亚家族之一。确定了cvcA的转录起始位点。伴刀豆球蛋白基因在发育中的豌豆种子子叶中表达，开花后16 - 18天相应mRNA种类的水平最高。基因序列显示伴刀豆球蛋白与豌豆球蛋白相似，但在蛋白质N端附近插入了一个121个氨基酸的序列。这个插入序列非常亲水，带有电荷和酸性残基的比例很高；其氨基酸组成与豌豆豆球蛋白基因α亚基C端附近的序列相似，但与它们没有直接的同源性。将该序列与大豆（Glycine max）伴大豆球蛋白（一种同源的豌豆球蛋白型蛋白）中的“插入”序列进行比较，表明这两个插入事件是独立发生的。该基因的5'侧翼序列除了一个共有启动子序列外，还包含几个推定的调控元件。
