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豌豆（Pisum sativum）种子脂氧合酶的cDNA克隆。两种主要豌豆种子脂氧合酶同工型的序列比较。

The cDNA cloning of a pea (Pisum sativum) seed lipoxygenase. Sequence comparisons of the two major pea seed lipoxygenase isoforms.

作者信息

Ealing P M, Casey R

机构信息

John Innes Institute, Norwich, U.K.

出版信息

Biochem J. 1989 Dec 15;264(3):929-32. doi: 10.1042/bj2640929.

DOI:10.1042/bj2640929
PMID:2515855
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1133675/
Abstract

Cloning and sequencing of two cDNAs from mRNA of maturing pea (Pisum sativum) seeds allowed the deduction of the complete amino acid sequence of a lipoxygenase polypeptide which is most similar to that of soya-bean lipoxygenase 2. The predicted Mr of this polypeptide is 97134, and its sequence permits comparisons between the lox2-type and the lox3-type lipoxygenase isoforms from pea and soya bean (Glycine max).

摘要

对成熟豌豆（Pisum sativum）种子mRNA中的两个cDNA进行克隆和测序，从而推导一种脂氧合酶多肽的完整氨基酸序列，该序列与大豆脂氧合酶2最为相似。此多肽预测的相对分子质量为97134，其序列可用于比较豌豆和大豆（Glycine max）中lox2型和lox3型脂氧合酶同工型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44a7/1133675/a9277bf41478/biochemj00193-0296-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44a7/1133675/4d6001b3d16f/biochemj00193-0296-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44a7/1133675/a9277bf41478/biochemj00193-0296-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44a7/1133675/4d6001b3d16f/biochemj00193-0296-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44a7/1133675/a9277bf41478/biochemj00193-0296-b.jpg

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本文引用的文献

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Proc Natl Acad Sci U S A. 1988 Jan;85(2):416-20. doi: 10.1073/pnas.85.2.416.
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Molecular cloning and primary structure of human 15-lipoxygenase.人15-脂氧合酶的分子克隆与一级结构
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Molecular analysis of a null mutant for pea (Pisum sativum L.) seed lipoxygenase-2.豌豆（Pisum sativum L.）种子脂氧合酶-2无效突变体的分子分析
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