Postgraduate Program in Health Science, HealthSciences Department, State University of Maringá (UEM), Paraná, Brazil.
Postgraduate Program in Health Science, HealthSciences Department, State University of Maringá (UEM), Paraná, Brazil.
Acta Trop. 2021 Oct;222:106019. doi: 10.1016/j.actatropica.2021.106019. Epub 2021 Jun 19.
This study detected and compared the levels of Il-17A, IFN-gamma and IL-10 in the amniotic fluid (AF) and serum of pregnant women with acute toxoplasmosis in southern Brazil. It also compared the serum levels of these mediators in pregnant women with acute or chronic toxoplasmosis and with uninfected women. The serological investigations of anti-T. gondii IgM and IgG from the 60 pregnant women were determined by chemiluminescence microparticle immunoassay (CMIA). Twenty patients were uninfected, twenty were in the chronic phase and twenty were in the acute phase of toxoplasmosis. The 20 pregnant women in acute phase all agreed with amniocentesis. Serum and AF cytokines were evaluated by sandwich enzyme-linked immunosorbent assay. The analyzed cytokines showed no significant difference in blood versus amniotic fluid levels of pregnant women in the acute toxoplasmosis. Furthermore, we observed that serum IL-17A was significantly higher in pregnant women in the acute phase of infection compared to pregnant women with chronic toxoplasmosis and seronegative pregnant women. T. gondii DNA was not amplified in any of the samples of amniotic fluid by the nested-PCR reaction. Serum IL-10 levels were also higher in negative pregnant women than in infected pregnant women. Our findings indicate the activation of an inflammatory response to infection by T. gondii and suggest that increased production of IL-17A may be a protective factor against infection of the fetus.
本研究检测并比较了巴西南部急性弓形虫病孕妇羊水中和血清中的 Il-17A、IFN-γ 和 IL-10 水平。还比较了急性或慢性弓形虫病孕妇和未感染孕妇血清中这些介质的水平。通过化学发光微粒子免疫分析（CMIA）对 60 名孕妇的抗 T. gondii IgM 和 IgG 进行血清学调查。20 名患者未感染，20 名处于慢性期，20 名处于急性感染期。20 名急性感染的孕妇均同意羊膜穿刺术。通过夹心酶联免疫吸附试验评估血清和 AF 细胞因子。分析的细胞因子显示，急性弓形虫病孕妇血液与羊水之间的水平没有明显差异。此外，我们观察到，与慢性弓形虫病孕妇和血清阴性孕妇相比，急性感染期孕妇血清中的 IL-17A 显著升高。巢式 PCR 反应未在任何羊水样本中扩增出 T. gondii DNA。阴性孕妇的血清 IL-10 水平也高于感染孕妇。我们的研究结果表明弓形虫感染引发了炎症反应，并且增加的 IL-17A 产生可能是针对胎儿感染的保护因素。
