Ministry of Science and Technology, Water and Environment Directorate, Iraq.
Department of Biology, Collage of Science /Baghdad University, Iraq.
Exp Parasitol. 2022 Mar;234:108217. doi: 10.1016/j.exppara.2022.108217. Epub 2022 Jan 25.
Cytokines are a group of immunomodulatory proteins leading to a variety of immune reactions in the human; these cytokines play a significant role in the development of appropriate immune responses against T. gondii. This study aims to reveal the association of toxoplasmosis with serum levels of IL-3, IL-17A, and IL-27 in aborted women. The blood samples of patients and controls were collected from Al-Alawiya Maternity Teaching Hospital/Baghdad/Iraq from 2019 to 2020 for detecting anti-T. gondii antibodies (IgG and IgM) and the level of interleukins by ELISA. The results of TORCH by rapid test for recurrent abortion recorded 25.3% seropositive for anti-Toxoplasma antibodies, and 31.5% seropositive for one or more cases of TORCH test (Cytomegalovirus, Rubella, and Herpes). Whereas the results for anti-T. gondii IgG and IgM antibodies were shown elevated positivity percentages by ELISA test; these percentages were 56.2% in recurrent abortion women with significant differences (P < 0.05). The results suggested that the IL-3 serum concentration of pregnant women, recurrent abortion, and recurrent abortion with toxoplasmosis was declined versus healthy women with significant differences (p < 0.05). However, the results revealed that the concentration of IL-17A in recurrent abortion, and recurrent abortion with toxoplasmosis elevated versus healthy women and pregnant women with significant difference (p < 0.05). Whereas the results indicated that the IL-27 serum concentration elevated with significant differences in recurrent abortion with toxoplasmosis group compared to healthy women, pregnant women, and recurrent abortion. Interestingly, the serum levels for IL-27 increased comparing to the levels of IL-3 and IL-17A in all groups with significant differences (P < 0.05). In conclusion, it appeared in this study that the role of IL-3, IL-17A, and IL-27 in the maternal immune response during infections can lead to abortion.
细胞因子是一组免疫调节蛋白，在人体中导致各种免疫反应；这些细胞因子在针对 T. gondii 的适当免疫反应的发展中起着重要作用。本研究旨在揭示弓形虫病与流产妇女血清中 IL-3、IL-17A 和 IL-27 水平的关系。从 2019 年至 2020 年，从伊拉克巴格达的 Al-Alawiya 妇产教学医院采集患者和对照者的血液样本，通过 ELISA 检测抗 T. gondii 抗体（IgG 和 IgM）和白细胞介素水平。快速检测 TORCH 对复发性流产的结果记录了 25.3%的抗弓形虫抗体血清阳性，31.5%的 TORCH 检测（巨细胞病毒、风疹和疱疹）呈一种或多种阳性。然而，ELISA 检测显示抗 T. gondii IgG 和 IgM 抗体的阳性百分比升高；复发性流产妇女的这些百分比为 56.2%，具有显著差异（P<0.05）。结果表明，与健康妇女相比，孕妇、复发性流产和弓形虫病复发性流产妇女的血清 IL-3 浓度下降，差异具有统计学意义（p<0.05）。然而，结果显示，与健康妇女和孕妇相比，复发性流产和弓形虫病复发性流产妇女的 IL-17A 浓度升高，差异具有统计学意义（p<0.05）。然而，结果表明，与健康妇女、孕妇和复发性流产相比，弓形虫病复发性流产组的血清 IL-27 浓度升高，差异具有统计学意义。有趣的是，与所有组中的 IL-3 和 IL-17A 水平相比，血清 IL-27 水平升高，差异具有统计学意义（P<0.05）。总之，本研究表明，IL-3、IL-17A 和 IL-27 在感染期间母体免疫反应中的作用可能导致流产。
