Alnisani Salah, Rebai Ahmed
Laboratory of Molecular and Cellular Screening Processes, Center of Biotechnology of Sfax, Sfax, Tunisia.
Faculty of Science of Sfax, University of Sfax, Sfax, Tunisia.
Iran J Parasitol. 2025 Apr-Jun;20(2):307-315. doi: 10.18502/ijpa.v20i2.19050.
We aimed to investigate the presence of thyroid peroxidase antibodies (anti-TPO) in women with spontaneous abortion, both with and without toxoplasmosis. This was achieved by evaluating and comparing the serum levels of interleukins IL-4, IL-6, IL-10, IL-17, and TNF-α in women who experienced abortion due to toxoplasmosis, categorized by positive and negative anti-TPO status, with those who were toxoplasmosis-negative and also negative for anti-TPO.
We evaluated the serological presence of IgG and IgM antibodies to using an ELISA method, in Samarra City, Salah al-Din Governorate, Iraq in 2021-2022. A sample of 153 women with spontaneous abortion were enrolled. We also measured the serum levels of interleukins IL-4, IL-6, IL-10, IL-17, TNF-α, and anti-TPO using the same technique.
Overall, 103 were -positive. Of these, 14 had positive anti-TPO results (13.5%), compared to only 3 positive cases among the 50 matched controls who were -negative (6.0%). The difference between -positive and -negative women regarding anti-TPO status was statistically significant for interleukins IL-4 (=0.010), IL-6 (=0.017), and IL-10 (=0.003), but not for IL-17 or TNF-α. Additionally, the statistical analysis revealed a highly significant difference in the average concentrations of interleukins IL-4 (=0.013) and IL-10 (<0.001) between the -positive/anti-TPO-positive group and the -negative/anti-TPO-negative group of aborted women.
Elevated concentrations of IL-4, IL-10, and IL-6 have been associated with women undergoing recurrent miscarriages and negative anti-TPO results. The complex interaction between pro-inflammatory and anti-inflammatory cytokines is essential for immunological balance and pregnancy outcomes in a condition of toxoplasmosis. An increase in IL-4 and IL-10 levels in anti-TPO-positive individuals may lead to an imbalance in immune response, facilitating the development of autoimmune thyroid disease.
我们旨在调查患有和未患有弓形虫病的自然流产女性体内甲状腺过氧化物酶抗体（抗-TPO）的存在情况。这是通过评估和比较因弓形虫病而流产的女性（根据抗-TPO状态分为阳性和阴性）与弓形虫病阴性且抗-TPO也为阴性的女性的白细胞介素IL-4、IL-6、IL-10、IL-17和肿瘤坏死因子-α（TNF-α）的血清水平来实现的。
2021年至2022年期间，在伊拉克萨拉赫丁省萨迈拉市，我们采用酶联免疫吸附测定（ELISA）法评估了针对[此处原文缺失相关内容]的IgG和IgM抗体的血清学存在情况。纳入了153例自然流产女性样本。我们还使用相同技术测量了白细胞介素IL-4、IL-6、IL-10、IL-17、TNF-α和抗-TPO的血清水平。
总体而言，103例为[此处原文缺失相关内容]阳性。其中，14例抗-TPO结果为阳性（13.5%），相比之下，在50例匹配的[此处原文缺失相关内容]阴性对照中只有3例阳性（6.0%）。在白细胞介素IL-4（P = 0.010）、IL-6（P = 0.017）和IL-10（P = 0.
