Duangyod Thidarat, Rujanapan Narawadee, Champakam Sorraya, Charoensup Rawiwan
Department of Applied Thai Traditional Medicine, School of Integrative Medicine, Mae Fah Luang University, Chiang Rai, Thailand.
Medicinal Plants Innovation Center of Mae Fah Luang University, Mae Fah Luang University, Chiang Rai, Thailand.
J Adv Pharm Technol Res. 2021 Apr-Jun;12(2):185-189. doi: 10.4103/japtr.JAPTR_55_21. Epub 2021 Apr 27.
Red limestone is a mixture of turmeric ( L.) powder and limestone which is made from burning shells at high temperature. The yellow mixture turns to red color or deep orange because of the reaction between turmeric and calcium carbonate in limestone. Red limestone is traditionally used to treat many diseases such as abscess, cut wound and insect bite. The purpose of this study was to investigate anti-inflammatory activity and chemical constituents of red limestone. The chemical analysis of red limestone extract by liquid chromatography with tandem mass spectrometry revealed that red limestone consisted of alpha-turmerone and curcumanolide B as major components. These compounds were related with the chemical constituents in extract which is a main ingredient of red limestone. However, curcuminoids were not detected in red limestone extract. Cytotoxicity of red limestone extract was investigated. Macrophage cell lines (RAW 264.7) and human keratinocyte cell lines (HaCaT cells) were investigated cell viability using MTT assay. Red limestone extract was nontoxic to normal cells such as macrophage cells and human keratinocyte cells. Moreover, the inflammatory activity was detected nitric oxide (NO) secretion in RAW 264.7 cells. The result showed that the extracts inhibited NO in dose-dependent manner and IC was found to be 102.42 μg/ml. It suggested that red limestone extract had a potential for anti-inflammatory activity.
红石灰石是姜黄（L.）粉末和石灰石的混合物，石灰石是通过高温燃烧贝壳制成的。由于姜黄与石灰石中的碳酸钙发生反应，这种黄色混合物会变成红色或深橙色。传统上，红石灰石用于治疗多种疾病，如脓肿、割伤和蚊虫叮咬。本研究的目的是研究红石灰石的抗炎活性和化学成分。通过液相色谱-串联质谱对红石灰石提取物进行化学分析表明，红石灰石的主要成分是α-姜黄酮和莪术醇内酯B。这些化合物与红石灰石主要成分提取物中的化学成分有关。然而，在红石灰石提取物中未检测到姜黄素类化合物。研究了红石灰石提取物的细胞毒性。使用MTT法研究巨噬细胞系（RAW 264.7）和人角质形成细胞系（HaCaT细胞）的细胞活力。红石灰石提取物对巨噬细胞和人角质形成细胞等正常细胞无毒。此外，在RAW 264.7细胞中检测到炎症活性为一氧化氮（NO）分泌。结果表明，提取物以剂量依赖性方式抑制NO，IC50为102.42μg/ml。这表明红石灰石提取物具有抗炎活性的潜力。
