Periago J L, De Lucchi C, Gil A, Suárez M D, Pita M L
Department of Biochemistry and Molecular Biology, University of Granada, Spain.
Biochim Biophys Acta. 1988 Sep 2;962(1):66-72. doi: 10.1016/0005-2760(88)90096-3.
The fatty acid and cholesterol contents of tissue membranes are the determinants of membrane stability and functionality. This study was designed to evaluate the influence of a high monounsaturated fatty acid diet on the fatty acid composition of rat liver microsomes and on their cholesterol and lipid phosphorus content. Weanling animals were fed for 5 weeks with high fat diets containing olive oil or corn oil. Saturated fatty acids were increased and oleic acid decreased in microsomal total phospholipids and in the three major phosphoglycerides, phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI), of rats fed corn oil as compared to the olive oil group. The percentage of linoleic acid was higher in the corn oil group, but only for total phospholipids and PC. Linoleic and alpha-linolenic metabolites were significantly increased in total phospholipids of olive oil-fed animals with respect to those fed corn oil. These changes were responsible for the low unsaturation index found in microsomal phospholipids of the corn oil group. The diet did not affect the microsome cholesterol or the lipid phosphorus content. These results show that, in olive oil-fed rats, the cholesterol content and the degree of unsaturation of liver microsomes was similar to that observed in weanling animals; this probably suggests an adequate maintenance of functionality of membranes in olive oil-fed animals.
组织膜中的脂肪酸和胆固醇含量是膜稳定性和功能的决定因素。本研究旨在评估高单不饱和脂肪酸饮食对大鼠肝微粒体脂肪酸组成及其胆固醇和脂质磷含量的影响。将断奶动物用含橄榄油或玉米油的高脂肪饮食喂养5周。与橄榄油组相比，喂食玉米油的大鼠微粒体总磷脂以及三种主要磷酸甘油酯（磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）和磷脂酰肌醇（PI））中的饱和脂肪酸增加，油酸减少。玉米油组中亚油酸的百分比更高，但仅针对总磷脂和PC。与喂食玉米油的动物相比，喂食橄榄油的动物总磷脂中亚油酸和α-亚麻酸代谢物显著增加。这些变化导致玉米油组微粒体磷脂中不饱和指数较低。饮食对微粒体胆固醇或脂质磷含量没有影响。这些结果表明，在喂食橄榄油的大鼠中，肝微粒体的胆固醇含量和不饱和程度与断奶动物中观察到的相似；这可能表明喂食橄榄油的动物中膜功能得到了充分维持。
