Suárez A, Faus M J, Gil A
Department of Biochemistry and Molecular Biology, University of Granada, Spain.
Lipids. 1996 Mar;31(3):345-8. doi: 10.1007/BF02529883.
The fatty acid composition of heart, kidney, and lung was studied in weanling rats fed three diets differing in their polyunsaturated fatty acid content for 0, 2, and 4 wk. The first group had a 10% w/w fat semipurified diet which consisted of a mixture of olive oil (62.5%), soybean oil (11.1%), and refined coconut oil (26.4%) and provided 18:1n-9, 18:2n-6, and 18:3n-3 in similar amounts to a maternal human milk (diet HO). The second group received 7% of HO fat and 3% fish oil (0.4% 20:4n-6 and 5% 22:6n-3 of total fatty acids) (diet FO), and the third group was fed 7% HO fat, 1.5% of the same fish oil, and 1.5% of a purified pig brain phospholipid concentrate (0.6% 20:4n-6 and 3.5% 22:6n-3 of total fatty acids) (diet FO + BPL). The experimental diets increased tissue monounsaturated fatty acids in comparison with rats at weaning. Tissue lipid content of 20:4n-6 was increased and 22:6n-3 decreased in Group HO compared with weanling rats, whereas opposite changes were observed in Group FO. Feeding diet FO + BPL increased 22:6n:3 in tissue lipids compared with diet HO, and increased 20:4n-6 content in relation to diet FO. Our results indicate that rat heart, kidney, and lung are highly responsive to dietary n-3 and n-6 long-chain polyunsaturated fatty acids during postnatal life.
在断奶大鼠中研究了心脏、肾脏和肺的脂肪酸组成，这些大鼠被喂食三种多不饱和脂肪酸含量不同的日粮，持续0、2和4周。第一组大鼠食用10%（w/w）脂肪的半纯化日粮，该日粮由橄榄油（62.5%）、大豆油（11.1%）和精炼椰子油（26.4%）混合而成，提供的18:1n-9、18:2n-6和18:3n-3的量与母乳相似（日粮HO）。第二组大鼠摄入7%的HO脂肪和3%的鱼油（占总脂肪酸的0.4% 20:4n-6和5% 22:6n-3）（日粮FO），第三组大鼠喂食7%的HO脂肪、1.5%的同一种鱼油和1.5%的纯化猪脑磷脂浓缩物（占总脂肪酸的0.6% 20:4n-6和3.5% 22:6n-3）（日粮FO + BPL）。与断奶时的大鼠相比，实验日粮增加了组织中的单不饱和脂肪酸。与断奶大鼠相比，HO组大鼠组织脂质中20:4n-6的含量增加，22:6n-3的含量降低，而FO组则观察到相反的变化。与日粮HO相比，喂食日粮FO + BPL增加了组织脂质中22:6n:3的含量，并且相对于日粮FO增加了20:4n-6的含量。我们的结果表明，在出生后的生命过程中，大鼠的心脏、肾脏和肺对日粮中的n-3和n-6长链多不饱和脂肪酸具有高度反应性。
