Mayo Clinic, Jacksonville, FL, USA.
Methods Mol Biol. 2021;2348:113-121. doi: 10.1007/978-1-0716-1581-2_7.
Long noncoding RNAs (lncRNAs) are implicated in many physiological or disease processes and alterations in their expression may contribute to the development of various diseases. Accurate quantitation of lncRNA can be useful in measuring changes in expression in different settings such as in the circulation where the measurement of lncRNA may be useful as a biomarker of disease. However, the low levels of lncRNA expression require the use of highly sensitive detection technologies for accurate quantitation. Digital polymerase chain reaction (dPCR) is a sensitive method for absolute quantification of lncRNA and can be useful for measurement of gene expression when transcript levels are low. By providing a direct measurement without normalization, the use of dPCR may provide advantages for quantitation of low-abundance targets.
长链非编码 RNA（lncRNA）参与许多生理或疾病过程，其表达的改变可能导致各种疾病的发生。lncRNA 的准确定量在测量不同环境中的表达变化时很有用，例如在循环系统中，lncRNA 的测量可能作为疾病的生物标志物很有用。然而，lncRNA 表达水平低需要使用高度敏感的检测技术进行准确定量。数字聚合酶链反应（dPCR）是一种用于 lncRNA 绝对定量的敏感方法，当转录本水平较低时，它可用于测量基因表达。通过提供无需标准化的直接测量，dPCR 的使用可能为低丰度靶标的定量提供优势。
