Yan Irene K, Lohray Rishabh, Patel Tushar
Department of Transplantation, Mayo Clinic, Jacksonville, FL, USA.
Methods Mol Biol. 2018;1740:155-162. doi: 10.1007/978-1-4939-7652-2_12.
Cell-to-cell communication involves the release of biological molecules into the extracellular space and their uptake by recipient cells. These molecules include RNA that can modulate cellular signaling and biological processes. To study extracellular RNA, highly sensitive and precise methods for their detection are needed. Digital polymerase chain reaction (dPCR) can be a useful method for detecting and analyzing extracellular RNA. The sensitivity of digital PCR can exceed that of quantitative PCR for low abundance targets such as extracellular RNA.
细胞间通讯涉及生物分子释放到细胞外空间并被受体细胞摄取。这些分子包括能够调节细胞信号传导和生物过程的RNA。为了研究细胞外RNA，需要高灵敏度和精确性的检测方法。数字聚合酶链反应（dPCR）可能是检测和分析细胞外RNA的一种有用方法。对于低丰度靶标如细胞外RNA，数字PCR的灵敏度可能超过定量PCR。
