在兔子的小脑延髓池插入导管收集脑脊液，测量惊厥期间胍基化合物水平。

Measurement during convulsions of guanidino compound levels in cerebrospinal fluid collected with a catheter inserted into the cisterna magna of rabbits.

作者信息

Hiramatsu M, Edamatsu R, Fujikawa N, Shirasu A, Yamamoto M, Suzuki S, Mori A

机构信息

Department of Neurochemistry, Okayama University Medical School, Japan.

出版信息

Brain Res. 1988 Jul 5;455(1):38-42. doi: 10.1016/0006-8993(88)90111-4.

DOI:10.1016/0006-8993(88)90111-4

PMID:3416191

Abstract

An easy method for collecting cerebrospinal fluid (CSF) was developed using a catheter inserted into the cisterna magna of rabbits. Levels of guanidino compounds in CSF collected by this method were measured. Levels of guanidinoacetic acid and creatinine increased at the onset of a pentylenetetrazol (PTZ)-induced convulsion. These levels normalized 2 h after the convulsion. The arginine level started to decrease 2 h after the convulsion, continued to decrease gradually and normalized 4 days after the convulsion. These results suggest that guanidinoacetic acid and creatinine are related to the initiation of PTZ-induced convulsions, and that 4 days are required for the metabolism of guanidino compounds to return to normal after a convulsion in rabbits.

摘要

通过将导管插入兔枕大池，开发了一种采集脑脊液（CSF）的简便方法。对用该方法采集的脑脊液中胍基化合物水平进行了测量。在戊四氮（PTZ）诱发惊厥发作时，胍基乙酸和肌酐水平升高。惊厥发作2小时后这些水平恢复正常。精氨酸水平在惊厥发作2小时后开始下降，继续逐渐下降，并在惊厥发作4天后恢复正常。这些结果表明，胍基乙酸和肌酐与PTZ诱发惊厥的起始有关，并且兔惊厥发作后胍基化合物代谢恢复正常需要4天时间。