Gradient of arginine vasopressin concentration but not angiotensin II concentration between cerebrospinal fluid of anterior 3rd ventricle and cisterna magna in dogs.

Dogs were chronically implanted with two devices for cerebrospinal fluid (CSF) sampling from (a) the anterior part of the 3rd ventricle and (b) the cisterna magna. In conscious dogs arginine vasopressin (AVP) concentration of CSF samples collected at different occasions were 2-3 times higher in the CSF of the 3rd ventricle as compared to the AVP concentration of the cisterna magna. Inhalation anesthesia stimulated AVP release into the CSF at both sites by a factor of about 2, the gradient between 3rd ventricle and cisterna magna CSF of 2-3 remained for AVP in simultaneously collected samples. In contrast, angiotensin II-like immunoreactivity of CSF was not significantly different at both sites, neither in the conscious dogs nor during anesthesia. It is concluded that the main amount of AVP enters the CSF at the 3rd ventricular level.