Kusaka Gen, Calvert John W, Smelley Christopher, Nanda Anil, Zhang John H
Department of Neurosurgery, Louisiana State University Health Sciences Center-Shreveport, 1501 Kings Highway, P.O. Box 33932, Shreveport, LA 71130-3932, USA.
J Neurosci Methods. 2004 May 30;135(1-2):121-7. doi: 10.1016/j.jneumeth.2003.12.013.
Monitoring cerebrospinal fluid pressure or intracranial pressure (ICP) is crucial in the study of neurosurgical disorders. In the present study, we report a new lumbar method for monitoring ICP in rats.
A PE10 catheter connected to a pressure transducer was placed into the subarachnoid space of L5 through the duramater after laminectomy to record lumbar cerebrospinal fluid pressure (lumbar-ICP). ICP at the cisterna magna (cisterna-ICP) was recorded simultaneously via a catheter in the subarachnoid space at the cisterna magna. Eighteen anesthetized adult male S-D rats were subjected to baseline recording followed by either experimental subarachnoid hemorrhage (SAH) induced by intravascular puncture method or experimental intracerebral hemorrhage (ICH) induced by blood injection with a stereotaxic system.
Baseline lumbar-ICP and cisterna-ICP varied between 6 and 8 mmHg, and respiratory variation could be detected. A similar acute response to SAH was recorded in both the lumbar-ICP and cisterna-ICP in all rats. In rats subjected to SAH, the lumbar catheter continuously and accurately monitored lumbar-ICP, and reliable pressure tracings were obtained for up to 24 h after SAH. However, continued cisterna-ICP monitoring was abandoned in two rats in the cisterna magna method due to obstruction of the catheter by blood clots (hematoma).
This new lumbar-ICP method is simple, safe, easy, and reliable in rats. Continued lumbar-ICP measurements provided monitoring for up to 24 h after experimental manipulation.
监测脑脊液压力或颅内压（ICP）在神经外科疾病研究中至关重要。在本研究中，我们报告一种在大鼠中监测ICP的新的腰椎方法。
在椎板切除术后，将连接压力传感器的PE10导管经硬脑膜置入L5蛛网膜下腔以记录腰段脑脊液压力（腰段ICP）。同时通过置于枕大池蛛网膜下腔的导管记录枕大池处的ICP（枕大池ICP）。18只麻醉的成年雄性S-D大鼠先进行基线记录，然后采用血管穿刺法诱导实验性蛛网膜下腔出血（SAH）或用立体定向系统注射血液诱导实验性脑出血（ICH）。
基线腰段ICP和枕大池ICP在6至8 mmHg之间变化，且可检测到呼吸变化。所有大鼠的腰段ICP和枕大池ICP对SAH均记录到类似的急性反应。在SAH大鼠中，腰段导管持续且准确地监测腰段ICP，SAH后长达24小时可获得可靠的压力描记图。然而，在枕大池方法中，有两只大鼠因导管被血凝块（血肿）阻塞而放弃持续的枕大池ICP监测。
这种新的腰段ICP方法在大鼠中简单、安全、易行且可靠。实验操作后持续的腰段ICP测量可提供长达24小时的监测。
