转录组谱分析发育中的叶片和茎尖，以揭示负责小麦抽穗期的分子机制和共表达基因。
Transcriptome profiling of developing leaf and shoot apices to reveal the molecular mechanism and co-expression genes responsible for the wheat heading date.
机构信息
The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, 100081, Beijing, China.
Hebei sub-center of National Maize Improvement Center of China, Key Laboratory of Crop Germplasm Resources of Northern China (Ministry of Education), College of Agronomy, Hebei Agricultural University, 071001, Baoding, China.
出版信息
BMC Genomics. 2021 Jun 23;22(1):468. doi: 10.1186/s12864-021-07797-7.
BACKGROUND
Wheat is one of the most widely planted crops worldwide. The heading date is important for wheat environmental adaptability, as it not only controls flowering time but also determines the yield component in terms of grain number per spike.
RESULTS
In this research, homozygous genotypes with early and late heading dates derived from backcrossed progeny were selected to conduct RNA-Seq analysis at the double ridge stage (W2.0) and androgynous primordium differentiation stage (W3.5) of the leaf and apical meristem, respectively. In total, 18,352 differentially expressed genes (DEGs) were identified, many of which are strongly associated with wheat heading date genes. Gene Ontology (GO) enrichment analysis revealed that carbohydrate metabolism, trehalose metabolic process, photosynthesis, and light reaction are closely related to the flowering time regulation pathway. Based on MapMan metabolic analysis, the DEGs are mainly involved in the light reaction, hormone signaling, lipid metabolism, secondary metabolism, and nucleotide synthesis. In addition, 1,225 DEGs were annotated to 45 transcription factor gene families, including LFY, SBP, and MADS-box transcription factors closely related to flowering time. Weighted gene co-expression network analysis (WGCNA) showed that 16, 336, 446, and 124 DEGs have biological connections with Vrn1-5 A, Vrn3-7B, Ppd-1D, and WSOC1, respectively. Furthermore, TraesCS2D02G181400 encodes a MADS-MIKC transcription factor and is co-expressed with Vrn1-5 A, which indicates that this gene may be related to flowering time.
CONCLUSIONS
RNA-Seq analysis provided transcriptome data for the wheat heading date at key flower development stages of double ridge (W2.0) and androgynous primordium differentiation (W3.5). Based on the DEGs identified, co-expression networks of key flowering time genes in Vrn1-5 A, Vrn3-7B, WSOC1, and Ppd-1D were established. Moreover, we discovered a potential candidate flowering time gene, TraesCS2D02G181400. Taken together, these results serve as a foundation for further study on the regulatory mechanism of the wheat heading date.
背景
小麦是世界上种植最广泛的作物之一。抽穗期对小麦的环境适应性很重要，因为它不仅控制着开花时间，还决定了穗粒数等产量构成。
结果
本研究选用正反交后代的早、晚抽穗纯合基因型，分别在叶片和顶端分生组织的二棱期（W2.0）和雌雄同体原基分化期（W3.5）进行 RNA-Seq 分析。共鉴定到 18352 个差异表达基因（DEGs），其中许多与小麦抽穗期基因密切相关。GO 富集分析表明，碳水化合物代谢、海藻糖代谢过程、光合作用和光反应与开花时间调控途径密切相关。基于 MapMan 代谢分析，DEGs 主要参与光反应、激素信号转导、脂质代谢、次生代谢和核苷酸合成。此外，1225 个 DEGs 被注释到 45 个转录因子基因家族，包括与开花时间密切相关的 LFY、SBP 和 MADS 框转录因子。加权基因共表达网络分析（WGCNA）显示，16、336、446 和 124 个 DEGs 分别与 Vrn1-5A、Vrn3-7B、Ppd-1D 和 WSOC1 具有生物学联系。此外，TraesCS2D02G181400 编码一个 MADS-MIKC 转录因子，与 Vrn1-5A 共表达，表明该基因可能与开花时间有关。
结论
RNA-Seq 分析为小麦在二棱期（W2.0）和雌雄同体原基分化期（W3.5）这两个关键花发育阶段的抽穗日期提供了转录组数据。基于鉴定的 DEGs，建立了 Vrn1-5A、Vrn3-7B、WSOC1 和 Ppd-1D 中关键开花时间基因的共表达网络。此外，我们发现了一个潜在的候选开花时间基因 TraesCS2D02G181400。综上所述，这些结果为进一步研究小麦抽穗期的调控机制奠定了基础。
Zotero 插件