National Institute of Oceanography & Fisheries, Alexandria, Egypt.
Chemistry Department, Faculty of Science, Alexandria University, Alexandria, Egypt.
Environ Sci Pollut Res Int. 2021 Nov;28(43):60833-60841. doi: 10.1007/s11356-021-14958-4. Epub 2021 Jun 24.
In more than two-thirds of the diabetic foot ulcer (DFUs) cases, lower limb amputation of foot ulceration is caused by the infection. The role of transition a metal complex as a therapeutic compound is becoming increasingly important. In vitro, four groups of antibiotics and one sulfa drug were tested against diabetic foot resistant bacteria. Using three concentrations of two different prepared metal complexes: copper (Cu) and silver (Ag) - isoniazid (Iso) and nicotinamide (Nicot) were tested against diabetic foot isolates. Results revealed that β-lactam drugs (cephradine and piperacillin) showed the minimum averages of MIC 265 μg/ml against Gram-positive and Gram-negative isolates. Silver isoniazid (Iso-Ag-1) metal complex was selected depending on the maximum averages of MIC against both types of clinical isolates. The combination between β-lactams and Iso-Ag-1 showed maximum FICI averages of 0.24 for Gram-positive and 0.28 for Gram-negative. In addition, a combination between Iso-Ag-1 with squilla chitosan nanoparticles (CSSq-nAg) showed averages of synergistic index by 0.23 against Staphylococcus aureus and 0.13, 0.30, and 0.27 against E. coli, K. pneumoniae, and Ps. aeruginosa, respectively. Final formula of Iso-Ag-1+CSSq-nAg + β-lactams (cephradine and piperacillin) showed a synergistic effect at FICI = 0.044 and 0.047, against G+ve and -ve, respectively. These two combinations showed a slight toxicity against the water flea Daphnia magna by 3.49 and 3.6 ppm, respectively. Results suggest the use of Iso-Ag-1-CSSq-nAg as enhancing agent in combination with β-lactams as a blind therapy in pharmaceutical preparations.
在超过三分之二的糖尿病足溃疡（DFU）病例中，下肢溃疡的截肢是由感染引起的。过渡金属配合物作为治疗化合物的作用变得越来越重要。在体外，对四组抗生素和一种磺胺类药物进行了测试，以对抗糖尿病足耐药菌。使用两种不同制备的金属配合物的三种浓度：铜（Cu）和银（Ag）-异烟肼（Iso）和烟酰胺（Nicot）对糖尿病足分离株进行了测试。结果表明，β-内酰胺类药物（头孢菌素和哌拉西林）对革兰氏阳性和革兰氏阴性分离株的 MIC265μg/ml 的平均值最小。银异烟肼（Iso-Ag-1）金属配合物根据对两种类型临床分离株的 MIC 的最大平均值进行了选择。β-内酰胺类药物与 Iso-Ag-1 的组合对革兰氏阳性菌和革兰氏阴性菌的 FICI 平均值分别为 0.24 和 0.28。此外，Iso-Ag-1 与 squilla 壳聚糖纳米粒（CSSq-nAg）的组合对金黄色葡萄球菌的协同指数平均值为 0.23，对大肠杆菌、肺炎克雷伯菌和铜绿假单胞菌的协同指数平均值分别为 0.13、0.30 和 0.27。Iso-Ag-1+CSSq-nAg+β-内酰胺类药物（头孢菌素和哌拉西林）的最终配方在 FICI=0.044 和 0.047 时表现出协同作用，分别针对 G+ve 和 -ve。这两种组合对水蚤 Daphnia magna 的毒性略有，分别为 3.49 和 3.6ppm。结果表明，将 Iso-Ag-1-CSSq-nAg 用作增强剂与β-内酰胺类药物联合使用作为药物制剂中的盲目治疗。
