Zhang Jinzhou, Jin Yan, Cao Mengmeng, Deng Jiahua, Chang Yadi, Shi Mingyan, Miao Zhiguo
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, Henan, China.
Life Science College, Luoyang Normal University, Luoyang, Henan, China.
Front Vet Sci. 2023 Feb 16;10:1123002. doi: 10.3389/fvets.2023.1123002. eCollection 2023.
polysaccharide (CYP) has received attention in recent years owing to its positive nutritional and medicinal characteristics. Copper is an essential trace metal in animals, which plays an important role in iron absorption and hemoglobin synthesis. However, no published study has evaluated polysaccharide copper complex (CYP-Cu) as a dietary additive in broilers. This study was conducted to investigate the effects of dietary CYP-Cu on growth performance, immunity, and oxidative resistance in broilers. A total of 360 1-day-old 817 broiler chickens were randomly divided into 4 groups, with 3 replicates of 30 birds each and were fed a basal diet with the addition of 0 (control group), 0.02, 0.10, and 0.50 g/kg CYP-Cu. The feeding trial lasted 48 days. On day 28 and day 48, 6 broilers in each group were slaughtered, respectively. Then the parameters of growth and carcass, serum biochemistry, immunity, and antioxidation, and the expression level of hepatic antioxidative genes were investigated. The results showed that compared with the control group, the supplementation of dietary CYP-Cu could improve the indexes of the growth, carcass, serum biochemistry, immunity and oxidation resistance in broilers, such as average daily gain (ADG), the slaughter percentage (SP), semi-evisceration weight percentage (SEWP), eviscerated carcass weight percentage (EWP), breast muscle percentage (BMP), leg muscle percentage (LMP), serum albumin (ALB), high density lipoprotein (HDL), insulin-like growth factor I (IGF-I), triiodothyronine (T3), thyroxine (T4), growth hormone (GH), insulin (INS), immunoglobulin M (IgM), immunoglobulin G (IgG), immunoglobulin A (IgA), interleukin 2 (IL-2), interleukin 4 (IL-4), interleukin 6 (IL-6), complement 3 (C3), complement 4 (C4), total superoxide dismutase (T-SOD), total antioxidative capacity (T-AOC), glutathione peroxidase (GSH-Px), and glutathione s-transferase (GSH-ST); these parameters in the 0.10 g/kg CYP-Cu treated group were significantly increased ( < 0.05) in the total trial period, with the exceptions that feed conversion ratio (FCR) and serum low density lipoprotein (LDL), malondialdehyde (MDA) were decreased in the total trial period. In addition, the antioxidative gene mRNA expression of Nuclear factor E2-related factor 2 ( ), Superoxide dismutase 1 ( ), Superoxide dismutase 2 ( ), and Catalase () were upregulated in the liver ( < 0.05). These results indicated that the supplementation of dietary CYP-Cu improved the growth, immunity, and oxidation resistance of broilers, and the addition of 0.10 g/kg CYP-Cu in broiler diets is recommended, which suggests that CYP-Cu may be a promising green feed additive in the poultry industry.
近年来，多糖（CYP）因其积极的营养和药用特性而受到关注。铜是动物必需的微量金属，在铁吸收和血红蛋白合成中起重要作用。然而，尚未有已发表的研究评估多糖铜络合物（CYP-Cu）作为肉鸡日粮添加剂的效果。本研究旨在探讨日粮中添加CYP-Cu对肉鸡生长性能、免疫力和抗氧化能力的影响。总共360只1日龄的817肉鸡随机分为4组，每组3个重复，每个重复30只鸡，分别饲喂添加0（对照组）、0.02、0.10和0.50 g/kg CYP-Cu的基础日粮。饲养试验持续48天。在第28天和第48天，每组分别宰杀6只肉鸡。然后研究生长和胴体参数、血清生化指标、免疫指标和抗氧化指标，以及肝脏抗氧化基因的表达水平。结果表明，与对照组相比，日粮中添加CYP-Cu可提高肉鸡的生长、胴体、血清生化、免疫和抗氧化指标，如平均日增重（ADG）、屠宰率（SP）、半净膛重百分比（SEWP）、全净膛重百分比（EWP）、胸肌百分比（BMP）、腿肌百分比（LMP）、血清白蛋白（ALB）、高密度脂蛋白（HDL）、胰岛素样生长因子I（IGF-I）、三碘甲状腺原氨酸（T3）、甲状腺素（T4）、生长激素（GH）、胰岛素（INS）、免疫球蛋白M（IgM）、免疫球蛋白G（IgG）、免疫球蛋白A（IgA）、白细胞介素2（IL-2）、白细胞介素4（IL-4）、白细胞介素6（IL-6）、补体3（C3）、补体4（C4）、总超氧化物歧化酶（T-SOD）、总抗氧化能力（T-AOC）、谷胱甘肽过氧化物酶（GSH-Px）和谷胱甘肽S-转移酶（GSH-ST）；在整个试验期内，0.10 g/kg CYP-Cu处理组的这些参数显著升高（P<0.05），例外的是，整个试验期内饲料转化率（FCR）、血清低密度脂蛋白（LDL）、丙二醛（MDA）降低。此外，肝脏中核因子E2相关因子2（Nrf2）、超氧化物歧化酶1（SOD1）、超氧化物歧化酶2（SOD2）和过氧化氢酶（CAT）的抗氧化基因mRNA表达上调（P<0.05）。这些结果表明，日粮中添加CYP-Cu可提高肉鸡的生长、免疫力和抗氧化能力，建议在肉鸡日粮中添加0.10 g/kg CYP-Cu，这表明CYP-Cu可能是家禽业中一种有前景的绿色饲料添加剂。
