Cortes Yunnuen Zepeda, Valenzuela Loreto Margarita, Pena Eduardo Arturo Elizalde, Espana Sanchez Beatriz Liliana
IEEE Trans Nanobioscience. 2022 Jan;21(1):89-96. doi: 10.1109/TNB.2021.3092287. Epub 2021 Dec 31.
The process of disinfection of wastewater must use friendly materials with the environment that achieve the inhibition of bacterial growth, aiming to improve the quality of the water. In this study, electrospun nanocomposites CS (chitosan)/AgNPs (silver nanoparticles) was developed for wastewater disinfection through filtration. First, AgNPs were synthesized by a green synthesis method using aloe vera (Aloe Barbadensis Miller) extract as a reducing agent, and AgNO as metal precursor. AgNPs were characterized by UV-Vis spectroscopy with a SPR band at 420 nm. Transmission Electron Microscopy (TEM) demonstrates the formation of semispherical AgNPs with a diameter ca. 16 nm. The nanocomposites fibers CS/AgNPs were analyzed by scanning electron microscopy (SEM), with the presence of uniform fibers (diameter ca. 120 nm) and embedded AgNPs, determined by EDS and elemental mapping. Surface topography of CS/AgNPs fibers was evaluated by Atomic Force Microscopy (AFM), where the surface roughness of fibers is increased with the NPs content. Finally, the antibacterial activity (AA) of CS/AgNPs nanocomposites was evaluated against S. aureus and P. aeruginosa during 1 and 2 h of contact, obtaining an AA of 90 % in CS/AgNPs-2 mM after 2 h with P. aeruginosa. The fabrication of electrospun nanocomposites with silver nanoparticles represents a platform to the fabrication of multifunctional polymer membranes for wastewater disinfection.
废水消毒过程必须使用对环境友好的材料，以抑制细菌生长，从而提高水质。在本研究中，通过过滤开发了用于废水消毒的静电纺丝纳米复合材料CS（壳聚糖）/AgNPs（银纳米颗粒）。首先，采用绿色合成方法，以芦荟（库拉索芦荟）提取物为还原剂、硝酸银为金属前驱体来合成AgNPs。通过紫外可见光谱对AgNPs进行表征，其表面等离子体共振带在420nm处。透射电子显微镜（TEM）显示形成了直径约为16nm的半球形AgNPs。通过扫描电子显微镜（SEM）对纳米复合纤维CS/AgNPs进行分析，结果表明存在均匀的纤维（直径约为120nm）以及嵌入的AgNPs，通过能谱分析（EDS）和元素映射确定。利用原子力显微镜（AFM）评估CS/AgNPs纤维的表面形貌，发现纤维的表面粗糙度随纳米颗粒含量增加。最后，评估了CS/AgNPs纳米复合材料在接触1小时和2小时期间对金黄色葡萄球菌和铜绿假单胞菌的抗菌活性（AA），在与铜绿假单胞菌接触2小时后，CS/AgNPs - 2 mM的抗菌活性达到90%。含银纳米颗粒的静电纺丝纳米复合材料的制备为制造用于废水消毒的多功能聚合物膜提供了一个平台。
