Allizond Valeria, Banche Giuliana, Salvoni Matteo, Malandrino Mery, Cecone Claudio, Cuffini Anna Maria, Bracco Pierangiola
Department of Public Health and Pediatric Sciences, University of Torino, Via Santena 9, 10126 Turin, Italy.
Department of Chemistry, NIS Interdepartmental Centre, University of Torino, Via P. Giuria 7, 10125 Turin, Italy.
Polymers (Basel). 2023 Mar 16;15(6):1470. doi: 10.3390/polym15061470.
Nanofibers can play an important role in developing new kinds of medical applications. Poly(lactic acid) (PLA) and PLA/poly(ethylene oxide) (PEO) antibacterial mats containing silver nanoparticles (AgNPs) were prepared by a simple one-step electrospinning method that allows AgNPs to be synthesized simultaneously with the preparation of the electrospinning solution. The electrospun nanofibers were characterized by scanning electron microscopy, transmission electron microscopy and thermogravimetry, while silver release over time was monitored by inductively coupled plasma/optical emission spectroscopy. The antibacterial activity was tested against and by colony forming unit (CFU) count on agar after 15, 24 and 48 h of incubation. AgNPs were found to be mainly concentrated in the PLA nanofiber core, and the mats showed steady but slow Ag release in the short term; in contrast, AgNPs were uniformly distributed in the PLA/PEO nanofibers, which released up to 20% of their initial silver content in 12 h. A significant ( < 0.05) antimicrobial effect towards both tested bacteria, highlighted by a reduction in the CFU/mL counts, was observed for the nanofibers of PLA and PLA/PEO embedded with AgNPs, with a stronger effect exerted by the latter, confirming the more efficient silver release from these samples. The prepared electrospun mats may have good potential for use in the biomedical field, particularly in wound dressing applications, where a targeted delivery of the antimicrobial agent is highly desirable to avoid infections.
纳米纤维在开发新型医学应用中可发挥重要作用。通过一种简单的一步电纺丝方法制备了含有银纳米颗粒（AgNPs）的聚乳酸（PLA）和PLA/聚环氧乙烷（PEO）抗菌垫，该方法可使AgNPs在制备电纺丝溶液的同时进行合成。通过扫描电子显微镜、透射电子显微镜和热重分析对电纺纳米纤维进行了表征，同时通过电感耦合等离子体/光发射光谱法监测银随时间的释放情况。在孵育15、24和48小时后，通过琼脂上的菌落形成单位（CFU）计数来测试对[具体细菌1]和[具体细菌2]的抗菌活性。发现AgNPs主要集中在PLA纳米纤维核心中，并且垫子在短期内显示出稳定但缓慢的银释放；相比之下，AgNPs均匀分布在PLA/PEO纳米纤维中，在12小时内释放了其初始银含量的20%。对于嵌入AgNPs的PLA和PLA/PEO纳米纤维，观察到对两种测试细菌都有显著（<0.05）的抗菌效果，表现为CFU/mL计数减少，后者发挥的效果更强，证实了这些样品中银的释放更有效。所制备的电纺垫在生物医学领域可能具有良好的应用潜力，特别是在伤口敷料应用中，在该应用中非常需要有针对性地递送抗菌剂以避免感染。
