Department of Pharmacy, School of Medicine and Pharmacy, Huye Biotechnology Laboratory Complex, University of Rwanda, Gikondo, KK 737 Street, P.O. Box 4285, Kigali, Rwanda; Laboratory of Pharmacognosy, Center for Interdisciplinary Research on Medicines, University of Liege, Avenue Hippocrate 15, B36, B4000 Liège, Belgium.
University of Liege, Laboratory of Analytical Pharmaceutical Chemistry, Center for Interdisciplinary Research on Medicines, Avenue Hippocrate 15, B36, B4000 Liège, Belgium.
Fitoterapia. 2021 Sep;153:104974. doi: 10.1016/j.fitote.2021.104974. Epub 2021 Jun 22.
In Rwanda, the roots of Pentas longiflora Oliv. (Rubiaceae) have been used for a long time to treat Pityriasis versicolor. However, many people reported the use of leaves instead of roots. This research was conducted to compare the phytochemical composition and establish chromatographic methods for the standardization of roots and leaves extracts of P. longiflora. During this process, three new pentalongin glycosides (pentalonginoside A, pentalonginoside B, and pentalonginoside C) and two known glycosides of the same type (harounoside and clarinoside), as well as rutin, luteolin-7-rutinoside were isolated from methanol extract of leaves. In addition, pentalongin and psychorubrin, previously isolated from ethylacetate roots extract, were also identified in Pentas longiflora ethylacetate leaves extract. The presence of the antifungal compound pentalongin in leaves may explain the traditional use of leaves in the treatment of Pytiriasis versicolor. Furthermore, harounoside, psychorubrin, and pentalongin were selected as markers for HPLC fingerprints of MeOH extract. The accuracy and risk profile demonstrated the reliability of the validated method. In general, considerable variations of concentration in plant metabolites, including pentalongin, were observed between samples from different sites. The content in pentalongin (expressed as juglone) in collected samples ranged between 1.7 and 70.0 mg/100 g. The highest concentration (70.0 ± 17 mg/100 g) was registered in the cultivated samples from Mukoni. This important variation of pentalongin concentrations according to sampling sites, shows that in order to guarantee equivalent efficacy, finished products with P. longiflora should be standardized based on their pentalongin content.
在卢旺达，Pentas longiflora Oliv.（茜草科）的根已被长期用于治疗花斑癣。然而，许多人报告说使用的是叶子而不是根。这项研究旨在比较其根和叶的化学成分，并建立化学成分的色谱方法，以实现规范化。在此过程中，从甲醇提取物中分离出三种新的 pentalongin 糖苷（pentalonginoside A、pentalonginoside B 和 pentalonginoside C）和两种相同类型的已知糖苷（harounoside 和 clarinoside），以及芦丁、木犀草素-7-芦丁。此外，pentalongin 和 psychorubrin 以前从根的乙酸乙酯提取物中分离出来，也在 Pentas longiflora 乙酸乙酯叶提取物中被鉴定出来。叶子中存在抗真菌化合物 pentalongin 可能解释了传统上使用叶子治疗花斑癣的原因。此外，harounoside、psychorubrin 和 pentalongin 被选为 MeOH 提取物 HPLC 指纹图谱的标记物。验证方法的准确性和风险概况证明了其可靠性。一般来说，在不同地点采集的样本中，植物代谢物，包括 pentalongin，的浓度存在相当大的差异。收集样本中 pentalongin（以胡桃醌表示）的含量在 1.7 至 70.0mg/100g 之间。含量最高（70.0±17mg/100g）的样本来自 Mukoni 栽培的样本。pentalongin 浓度根据采样地点的这种重要变化表明，为了保证等效疗效，应根据其 pentalongin 含量对含有 P. longiflora 的成品进行标准化。
