Department of Biophysics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, CZ-783 71, Olomouc, Czech Republic.
Institute of Botany of the Czech Academy of Sciences, Department of Experimental and Functional Morphology, Dukelská135, CZ-379 82, Třeboň, Czech Republic.
Plant Physiol Biochem. 2021 Sep;166:459-465. doi: 10.1016/j.plaphy.2021.06.014. Epub 2021 Jun 16.
Terrestrial carnivorous plants of genera Drosera, Dionaea and Nepenthes within the order Caryophyllales employ jasmonates for the induction of digestive processes in their traps. Here, we focused on two aquatic carnivorous plant genera with different trapping mechanism from distinct families and orders: Aldrovanda (Droseraceae, Caryophyllales) with snap-traps and Utricularia (Lentibulariaceae, Lamiales) with suction traps. Using phytohormone analyses and simple biotest, we asked whether the jasmonates are involved in the activation of carnivorous response similar to that known in traps of terrestrial genera of Droseraceae (Drosera, Dionaea). The results showed that Utricularia, in contrast with Aldrovanda, does not use jasmonates for activation of carnivorous response and is the second genus in Lamiales, which has not co-opted jasmonate signalling for botanical carnivory. On the other hand, the nLC-MS/MS analyses revealed that both genera secreted digestive fluid containing cysteine protease homologous to dionain although the mode of its regulation may differ. Whereas in Utricularia the cysteine protease is present constitutively in digestive fluid, it is induced by prey and exogenous application of jasmonic acid in Aldrovanda.
石蒜科的茅膏菜属和猪笼草属以及车前科的露珠草属植物在其陷阱中诱导消化过程时使用茉莉酸。在这里，我们专注于两个具有不同的捕虫机制的水生食虫植物属，它们分别来自不同的科和目：具有弹片陷阱的狸藻属（狸藻科，石竹目）和具有吸捕器的狸藻属（狸藻科，唇形目）。我们使用植物激素分析和简单的生物测试来询问茉莉酸是否参与了食虫反应的激活，类似于已知的陆地狸藻科（茅膏菜属、猪笼草属）的陷阱中的食虫反应。结果表明，与狸藻属不同，狸藻属不使用茉莉酸来激活食虫反应，是唇形目中第二个没有利用茉莉酸信号进行植物性食虫的属。另一方面，nLC-MS/MS 分析表明，这两个属都分泌含有半胱氨酸蛋白酶的消化液，与猪笼草属中的猪笼草相似，尽管其调控模式可能不同。在狸藻属中，半胱氨酸蛋白酶在消化液中一直存在，但在露珠草属中，它是由猎物和外源茉莉酸诱导产生的。
