诱导幼斑马鱼炎症和急性髓鞘变性的方案。
Protocol for inducing inflammation and acute myelin degeneration in larval zebrafish.
机构信息
Ann Romney Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
出版信息
STAR Protoc. 2022 Jan 28;3(1):101134. doi: 10.1016/j.xpro.2022.101134. eCollection 2022 Mar 18.
This protocol details the induction of inflammation and acute myelin degeneration in larval zebrafish with a duration of <10 days. We describe the use of this model to screen the effects of candidate compounds on inflammation, followed by RNA isolation, and qPCR-based quantification of gene expression. We then outline the steps for bioinformatic analysis of the mechanisms associated with the compounds. This protocol can be used in combination with drugs and genetic targeting to identify pathways that contribute to neurodegeneration. For complete details on the use and execution of this profile, please refer to Wheeler et al. (2019).
本方案详细介绍了在幼鱼斑马鱼中诱导炎症和急性髓鞘变性的过程，总时长<10 天。我们描述了使用该模型筛选候选化合物对炎症的影响，然后进行 RNA 分离，并基于 qPCR 定量基因表达。接下来，我们概述了与化合物相关的机制的生物信息学分析步骤。该方案可与药物和基因靶向联合使用，以鉴定导致神经退行性变的途径。有关该方案的使用和执行的完整详细信息，请参考 Wheeler 等人。(2019)。
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