有氧运动对糖尿病大鼠心肌内皮组织中 mir-126 及其相关靶基因表达的影响。
The effect of aerobic exercise on the expression of mir-126 and related target genes in the endothelial tissue of the cardiac muscle of diabetic rats.
机构信息
Department of Exercise Physiology, Faculty of Sport Sciences, Urmia University, Urmia, Iran.
Department of Exercise Physiology, Faculty of Sport Sciences, Urmia University, Urmia, Iran.
出版信息
Microvasc Res. 2021 Nov;138:104212. doi: 10.1016/j.mvr.2021.104212. Epub 2021 Jun 24.
BACKGROUND
The aim of this study was to investigate the effect of 8 weeks of aerobic exercise on the expression of mir-126 and some angiogenesis factors in the endothelial tissue of the cardiac muscle of type 2 diabetic rats.
METHODS
Sixteen male Wistar rats were divided into two groups: diabetic control and diabetic training. Nicotinamide and streptozotocin injections were used to induce type 2 diabetes. After familiarization, the training group participated in an 8-week exercise protocol on a treadmill with an intensity of 25 m per minute, a slope of 5% and 30 min per session. RT-PCR was used to evaluate the expression of mir-126 and PI3K genes. Expression of raf1, VEGF, blood glucose and insulin was determined by ELISA and insulin resistance was assessed by HOMA-IR homeostasis model. Immunohistochemistry was used to measure the capillary density of the cardiac muscle. Data were analyzed by t-test for independent groups with a significance level of p < 0.05.
RESULTS
Diabetes reduces angiogenesis in cardiac tissue, which is associated with a significant reduction in the expression of mir-126, raf1, VEGF and PI3K; while aerobic exercise increased the expression of mir-126, raf1, PI3K, VEGF. Exercise also decreased blood glucose levels and insulin resistance.
CONCLUSION
It seems that aerobic exercise can prevent the destructive effects of diabetes by activating the angiogenic pathway of cardiac tissue. Therefore, regulatory processes through mir-126, which are influenced by aerobic exercise, can be a valuable strategy in developing new treatments for diabetes.
背景
本研究旨在探讨 8 周有氧运动对 2 型糖尿病大鼠心肌内皮组织中 mir-126 表达及部分血管生成因子的影响。
方法
将 16 只雄性 Wistar 大鼠随机分为 2 组：糖尿病对照组和糖尿病训练组。使用烟酰胺和链脲佐菌素注射诱导 2 型糖尿病。适应后，训练组在坡度为 5%、速度为 25m/min 的跑步机上进行 8 周运动方案，每次 30 分钟。采用 RT-PCR 法检测 mir-126 和 PI3K 基因的表达。采用 ELISA 法检测 raf1、VEGF 的表达，检测血糖和胰岛素，通过 HOMA-IR 稳态模型评估胰岛素抵抗。采用免疫组织化学法检测心肌毛细血管密度。数据采用独立样本 t 检验进行分析，p 值<0.05 为差异有统计学意义。
结果
糖尿病可降低心肌组织的血管生成，这与 mir-126、raf1、VEGF 和 PI3K 的表达显著降低有关；而有氧运动可增加 mir-126、raf1、PI3K、VEGF 的表达。运动还可降低血糖水平和胰岛素抵抗。
结论
有氧运动似乎可以通过激活心肌组织的血管生成途径来预防糖尿病的破坏性影响。因此，通过 mir-126 进行调节的过程可能是开发糖尿病新疗法的一种有价值的策略。
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