产碳青霉烯类抗生素的OA-6129阻断突变体的诱变及碳青霉烯类抗生素的生物合成。

Mutagenesis of OA-6129 carbapenem-producing blocked mutants and the biosynthesis of carbapenems.

作者信息

Kojima I, Fukagawa Y, Okabe M, Ishikura T, Shibamoto N

机构信息

Sanraku Incorporated, Central Research Laboratories, Fujisawa, Japan.

出版信息

J Antibiot (Tokyo). 1988 Jul;41(7):899-907. doi: 10.7164/antibiotics.41.899.

DOI:10.7164/antibiotics.41.899

PMID:3417564

Abstract

Streptomyces fulvoviridis A933 17M9 1501 is an A933 acylase-defective mutant derived from S. fulvoviridis A933 17M9 and thus produces the OA-6129 group of carbapenems and carbapenams. By further mutation of mutant 1501, 4 types of mutants (OA-6129 A + B1 + B2 producers; OA-6129 A + B2 producers; an OA-6129 A producer; non-producers) were obtained. The second type of mutant strains 4N 3607, 5NA 3949-40 and 5NE 252 proved useful for the fermentative production of carbapenem OA-6129 B2. These results of mutagenesis demonstrated that the sequence of carbapenem bioconversion in the horizontal route was hydroxylation at C-8----isomerization at C-6----sulfation at C-8 hydroxyl.

摘要

黄绿链霉菌A933 17M9 1501是源自黄绿链霉菌A933 17M9的A933酰基转移酶缺陷型突变体，因此可产生OA - 6129组碳青霉烯类和碳青霉烯胺类。通过对突变体1501的进一步诱变，获得了4种类型的突变体（OA - 6129 A + B1 + B2产生菌；OA - 6129 A + B2产生菌；OA - 6129 A产生菌；不产生菌）。第二类突变株4N 3607、5NA 3949 - 40和5NE 252被证明可用于碳青霉烯类OA - 6129 B2的发酵生产。这些诱变结果表明，水平途径中碳青霉烯生物转化的顺序为C - 8位羟基化→C - 6位异构化→C - 8位羟基硫酸化。